بررسی قابلیت‌های تکنولوژیکی و خصوصیات زیست فعال پلی ساکارید ساقه تیفا

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم و صنایع غذایی،دانشکده علوم زیستی،دانشگاه آزاد واحد تهران شمال،تهران،ایران

2 گروه علوم و صنایع غذایی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران

3 عضو هیئت علمی دانشگاه آزاد اسلامی واحداهواز

4 گروه علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه تربیت مدرس

5 گروه علوم و صنایع غذایى، دانشگاه علوم کشاورزى و منابع طبیعى خوزستان

چکیده

< p>این تحقیق با هدف جداسازی پلی ساکارید ساقه تیفا و بررسی خصوصیات تکنولوژیک، فعالیت ضد اکسایشی و ضد باکتریایی آن انجام شد. در این راستا پلی ساکارید ساقه تیفا با استفاده از روش استخراج قلیایی-اولتراسونیک تحت شرایط نسبت آب به ماده خام mL/g 25 ، غلظت سدیم هیدروکسیدM 5/1 و زمانmin 40 استخراج گردید. در این بررسی نتایج پراکندگی نور پویا نشان داد که متوسط اندازه ذرات و پتانسیل زتا به ترتیب nm 282 و mV 52/21- بود. بر اساس نتایج نسبت هاسنر و قابلیت جریان پلی ساکارید تیفا به ترتیب 02/0±16/1 و 01/0±99/13% بود. این ترکیب ظرفیت بالایی برای نگهداری آب (15/0±28/9 %)، جذب روغن(14/0±37/4%)، فعالیت امولسیونی (%100) و پایداری امولسیون(%96) نشان داد. همچنین نتایج بررسی فعالیت ضد اکسایشی بیانگر توانایی مطلوب پلی‌ساکارید تیفا ( mg/mL47/0= 〖IC〗_(50 )) برای مهار رادیکال‌های آزاد ABTS بود. نتایج بررسی فعالیت ضد باکتریایی به روش انتشار دیسک در آگار نشان داد که این ترکیب دارای تاثیر معنی داری بر مهار رشد باکتری های منتخب در این مطالعه نبود. با توجه به نتایج به دست آمده این پلی ساکارید می‌تواند به عنوان یک منبع طبیعی آنتی اکسیدان و ترکیبی مناسب برای استفاده های تکنولوژیکی معرفی گردد.

چکیده تصویری

بررسی قابلیت‌های تکنولوژیکی و خصوصیات زیست فعال پلی ساکارید ساقه تیفا

تازه های تحقیق

  • با استفاده از روش استخراج قلیایی-اولتراسونیک پلی ساکارید از ساقه تیفا دمینجنسیس جدا شد.
  • قابلیت­های تکنولوژیکی پلی ساکارید استخراح شده مورد بررسی قرار گرفت.
  • خصوصیات فیزیکی پلی ساکارید استخراج شده شامل اندیس های جریان و پیوستگی مورد بررسی قرار گرفت.
  • علیرغم فعالیت آنتی اکسیدانی مطلوب پلی ساکارید استخراج شده خصوصیت ضد باکتریایی علیه باکتریهای مورد مطالعه نشان نداد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Investigation of technological capabilities and bioactive properties of Typha stem polysaccharide

نویسندگان [English]

  • Reihaneh Sorourian 1
  • Amir Eghbal Khajehrahimi 2
  • Mohammad Hossein Azizi 4
  • Mohammad Hojjati 5
1 Department of Food Science and Technology, North Tehran Branch, Islamic Azad University, Tehran, Iran
2 Department of Food Science and Technology, North Tehran Branch, Islamic Azad University, Tehran, Iran.
3
4 Department of Food Science and Technology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
5 Department of Food Science and Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Ahvaz, Iran
چکیده [English]

< p >The aim of this study was to isolate polysaccharides from the Typha stem to evaluate its technological specifications and antibacterial and antioxidant activities. To isolate this polysaccharide, we used the alkaline- ultrasonic extraction method in this state; water to raw material ratio is 25 mL/g, sodium hydroxide concentration 1.5 M for 40 min. The dynamic light scattering results illustrated that the average particle size and zeta potential were 282 nm and -21.52 mV, respectively. Based on the results, the Hausner''s ratio and Carr’s Index were 1.16±0.02 and %13.99±0.01, respectively. This polysaccharide showed a high capacity for water holding (9.28±0.15), oil holding (4.37±0.14), Emulsifying (100%) and emulsion stability (96%). Also, the results of this study showed that the extracted polysaccharide has a high ability to scavenge free radicals ABTS (IC50=0.47 mg/mL). Examination of antibacterial activity by disk agar diffusion method showed that this compound did not have a significant effect, on inhibiting the growth of selected bacteria in this study. Therefore, this polysaccharide can be introduced as a natural source of antioxidant and an appropriate composition for technological uses.

کلیدواژه‌ها [English]

  • Polysaccharides
  • Typha stem
  • Technological capability
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