بررسی کمیت و انتشار نایسین استفاده شده در فیلم زئین ذرت بر کاهش جمعیت میکروکوکوس لوتئوس

نوع مقاله : مقاله پژوهشی

نویسنده

استادیار، گروه صنایع غذایی، دانشگاه آزاد اسلامی واحد ور امین - پیشوا

چکیده

نایسین یک باکتریوسین است که در سیستم‌های غذایی، فعالیت خود را به‌دلیل اتصال آن به پروتئین و چربی از دست می‌دهد بنابراین با تهیه یک فیلم بر پایه زیست پلیمر می‌توان فعالیت آن را بهبود بخشید. هدف از این پژوهش بررسی فعالیت ضد‌میکروبی و کمیت انتشار غلظت‌های مختلف نایسین 100، 200 و 300 میلی‌گرم، از فیلم زئین ذرت 4 درصد در بافر فسفات و ارزیابی فعالیت ضد‌میکروبی و کمیت نایسین انتشار یافته از غلظت‌های مختلف فیلم زئین ذرت (4، 6، 8 و 10 درصد) در بافر فسفات بود. فعالیت ضد‌میکروبی نایسین انتشار یافته از فیلم‌های زئین ذرت با بررسی مهار رشد میکروکوکوس لوتئوس در سطح محیط کشت انجام شد و کمیت آن نیز به روش کروماتوگرافی مایع با عملکرد بالا ارزیابی گردید. در مرحله آخر مدل‌سازی حرکتی انتشار نایسین به روش مدل‌سازی وایبل تجربی انجام شد. مطابق با نتایج، فعالیت ضد‌میکروبی نایسین با تشکیل هاله عدم رشد در آگار تایید گردید. کمیت انتشار نایسین از فیلم‌ زئین ذرت 4 درصد با افزایش غلظت نایسین به شکل معنی‌داری افزوده شد. با افزایش غلظت فیلم زئین ذرت از 4 به 10 درصد (وزنی/حجمی)، میزان انتشار نایسین از فیلم زئین ذرت 10 درصد به‌دلیل افزایش پیچ خوردگی در فیلم و رهایش کندتر نایسین 4 برابر کاهش یافت. مدل‌سازی حرکتی انتشار از فیلم‌ها مشابه رفتار فیک با پیش‌بینی عالی (95/0<2R) را نشان داد. بنابراین با استفاده از پوشش خوراکی زئین ذرت حاوی ترکیب ضد‌میکریی نایسین می‌توان زمان نگه‌داری و کیفیت مواد غذایی را افزایش داد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Study of Quantity and Diffusion of Nisin Used in Corn Zein Film to Depopulate Micrococcus Luteus

نویسنده [English]

  • Leila Nateghi
Assistant professor, Department of Food Science and Technology, Faculty of Agriculture, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran
چکیده [English]

Nisin is a bacteriocin that loses its activity due to binding to the protein, fat and other in the food systems. By preparing a film based on the biopolymer can improve its performance. The objective of this research was to investigate the antimicrobial activity and quantity diffusion of different concentrations of nisin (100, 200 and 300 mg) from 4 percent corn zein film in phosphate buffer and evaluation of antimicrobial activity and quantity diffusion of nisin from different concentrations of corn zein films (2, 4, 6, 8 and 10) in the phosphate buffer. The antimicrobial activity of nisin diffusion from corn zein films was performed with evaluation of growth inhibition of Micrococcus luteus in culture media and its quantity was evaluated with high-performance liquid chromatography method. In the final stage modeling of nisin release kinetics performed via empirical Weibull model. According to results the antimicrobial activity of nisin was confirmed by formation of growth inhibition halo in agar. Quantity diffusion of nisin from 4 percent corn zein film significantly increased with increasing concentration of nisin. With increasing the concentrations of corn zein from 4 to 10% (W/V) the nisin diffusion from corn zein film 10%, four times decreased which related to increase kink in film and slower release of nisin. Kinetic modeling diffusion from films showed similar behavior Fick with excellent prediction (R2 >0.95). Therefore by using the corn zein edible coating containing nicin antimicrobial composition can increase the shelf life and quality of food.

کلیدواژه‌ها [English]

  • Biodegradable biopolymer
  • Corn zein
  • Micrococcus luteus
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