ارزیابی اثر ژلاتین ماهی، کاپا کاراگینان و روشهای مختلف ریزپوشانی بر پایداری اکسایشی میکروکپسول‌های روغن ماهی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استاد، گروه فرآوری محصولات شیلاتی-دانشگاه علوم کشاورزی و منابع طبیعی گرگان

2 دانش آموخته دکترا، فرآوری محصولات شیلاتی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان

3 استادیار، فرآوری محصولات شیلاتی، دانشکده شیلات، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

4 دانشیار، گروه مهندسی مواد و طراحی صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان

چکیده

در این پژوهش پایداری اکسایشی روغن ماهی ریز پوشانی شده با دیواره های مختلف (ژلاتین ماهی، کاپا کاراگینان و ترکیبی) و روش‌های فیزیکی و شیمیایی (کوسرواسیون، خشک کردن پاششی و انجمادی) مورد بررسی قرار گرفت. میزان رهایش پودرهای ریزپوشانی شده در شرایط آزمایشگاهی و بررسی شاخص پراکسید و آنیسیدین طی 10 روز نگهداری در شرایط تشدید شده (45 درجه سانتی‌گراد) ارزیابی شد. نتایج بررسی میزان حلالیت پودرهای پروتئینی و پایداری اکسایشی نشان دادند که ژلاتین ماهی بهترین ترکیب دیواره و روش کوآسرواسیون نیز مناسب‌ترین روش برای ریزپوشانی روغن ماهی بود. به‌طوری‌که پودرهایی با میزان حلالیت بالاتر و پراکسید و آنیسیدین کمتر طی نگهداری را تولید نماید. بیشترین و کمترین میزان ترکیب هسته رها شده در پودرهای تهیه شده با روش کوسرواسیون مشاهده گردید (16/93 و 80 درصد). دیواره ژلاتینی میزان رهایش بیشتری را در مقایسه با سایر دیواره‌ها در روش خشک‌کن پاششی و خشک‌کن انجمادی داشت. مقایسه سه روش تولیدی و مواد دیواره نشان داد که ترکیب دیواره و نوع روش تولیدی با تاثیر بر خواص فیزیکی، روی پایداری روغن ماهی ریزپوشانی شده موثر می‌باشد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Fish gelatin and κ carrageenan and different encapsulation methods on oxidative stability of fish oil microcapsules

نویسندگان [English]

  • Bahare Shabanpour 1
  • Bahar Mehard 2
  • Parastoo Pourashouri 3
  • Seyed Mahdi Jafari Jafari 4
1 Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
2 Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
3 Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
4 Department of Food Process Engineering, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
چکیده [English]

In this study the stability and release of microencapsulated fish oil prepared by different wall materials (fish gelatin, κ -carrageenan and fish gelatin+ κ carrageenan), physical and chemical methods (coacervation, spray drying and freeze drying) were examined. In-vitro controlled release of the microcapsules and peroxide (PV); ρ-Anisidine values during 10 days storage at accelerated conditions (45 °C) were investigated. The results of solubility of protein powders and oxidative stability showed that fish gelatin was the best wall material and coacervation was the best method to encapsulation of fish oil. These powders had higher solubility and lower PV, p-AV than the other treatments. The highest and lowest core releases were observed by coacervation method (93.16 and 80 %). At spray and freeze drying methods, gelatin had more release than the other wall materials. Comparison of the different methods and wall materials confirmed that combination of matrices, drying methods are effective on physical characteristics and oxidative stability of encapsulated fish oil.

کلیدواژه‌ها [English]

  • Coacervation
  • Controlled release
  • Encapsulation
  • Fish oil
  • Fish gelatin
  • Spray and freeze drying
 [1] Kagami, S., Sugimura, S., Fujishima, N., Matsuda, K., Kometani, T., Matsumura, Y. (2003). Oxidative stability, structure, and physical characteristics of microcapsules formed by spraying drying of fish oil with protein and dextrin wall materials. J. Food Sci., 68, 2248–2255.
 
[2] Cho, Y. H., Shim, H. K., Park, J. (2003). Encapsulation of fish oil by an enzymatic gelation process using transglutaminase cross-linked proteins. J. Food Sci., 68, 2717–2723.
 
[3] Sioen, I. A., Pynaert, I., Matthys, C., De Backer, G., Van Camp, J., De Henauw, S. (2006). Dietary intakes and food sources of fatty acids for Belgian women, focused on n-6 and n-3 polyunsaturated fatty acids. Lipids, 41, 415–422.
 
 [4] Poyato, C., Ansorena, D., Berasategi, I., Navarro-Blasco, I., Astiasaraan, I. (2014). Optimization of a gelled emulsion intended to supply omega-3 fatty acids into meat products by means of response surface methodology. Meat Sci, 98(4), 615–621.
 
[5] Jacobsen, C. (1999). Sensory impact of lipid oxidation in complex food systems. Lipid / Fett., 101, 484–492.
 
[6] Jiménez-Martín, E., Pérez-Palacios, T., Carrascal, J.R. Rojas, T.A. (2015). Enrichment of chicken nuggets with microencapsulated Omega-3 fish oil: effect of frozen storage time on oxidative stability and sensory quality. Food Bioproc. Tech., DOI 10.1007/s11947-015-1621-x.
 
 [7] Eratte, D., Wang, B., Dowling, K., Barrow C. J., Adhikari, B.P. (2014). Complex coacervation with whey protein isolate & gum arabic for the microencapsulation of omega-3 rich tuna oil. Food Function, 5, 2743- 2750.
 
[8] Drusch, S., Berg, S. (2008). Extractable oil in microcapsules prepared by spray-drying: localisation, determination and impact on oxidation stability. Food Chem., 109, 17–24.
 
 [9] Shaw, L. A., McClements, D. J., Decker, E. A. (2007). Spray-dried multilayered emulsions as a delivery method for omega-3 fatty acids into food systems. J. Agric. Food Chem., 55(8), 3112–3119.
 
[10] Pourashouri, P., Shabanpour, B., Razavi, S. H., Jafari, S. M., Shabani, A., Aubourg, S. 2014. Impact of wall materials on physicochemical properties of microencapsulated fish oil by spray drying. Food Bioproc. Tech, 51, 348–355.
 
[11]Shi, L.E., Li, Z.H., Zhang, Z.L., Zhang, T.T., Yu, W.M., Zhou, M.L., Tang, Z.X. (2013). Encapsulation of Lactobacillus bulgaricus in carrageenan-locust bean gum coated milk microspheres with double layer structure. LWT - Food Sci. Technol., 54 (1), 147–151.
 
[12] Galazka, V.B., Dickinson, E., Ledward, D.A. (1999). Emulsifying behavior of globulin Vicia faba in mixtures with sulphated polysaccharides: Comparison of thermal and high-pressure treatments. Food Hydrocolloids, 13, 425–435.
 
[13] Lobo, L. (2002). Coalescence during emulsification; 3. Effect of gelatin on rupture and coalescence. J. Colloid Interface Sci., 254,165–174.
 
[14] Karim, A.A., Bhat, R. (2009). Fish gelatin: properties, challenges, and prospects as an alternative to mammalian gelatins. Food Hydrocoll., 23, 563–576.
 
 [15] Garcia, E., Gutierrez, S., Nolasco, H., Carreon, L., Arjona, O. (2006) Lipid composition of shark liver oil: effects of emulsifying and microencapsulation processes. Eur Food Res Technol., 222, 697–701.
 
[16] Aghbashlo, M., Mobli, H., Madadlou, A., Rafiee, S. (2012). Influence of wall material and inlet drying air temperature on the microencapsulation of fish oil by spray drying. Food Bioproc. Technol., doi:10.1007/s11947-012-0796-7.
[17] Drusch, S., Serfert, Y., Berger, A., Shaikh, M.Q., Rätzke, K., Zaporojtchenko, V., Schwarz, K. (2012). New insights into the microencapsulation properties of sodium caseinate and hydrolyzed casein. Food Hydrocolloids, 27, 332-338.
 
[18] Lim, H.K., Tan, C.P., Bakar, J., Ng, S.P. (2011). Effects of different wall materials on the physicochemical properties and oxidative stability of spray-dried microencapsulated red-fleshed pitaya (Hylocereus polyrhizus) Seed Oil. Food Bioproc. Technol., DOI 10.1007/s11947-011-0555-1.
 
[19] Gallardo, G., Guida, L., Martinez, V., López, M. C., Bernhardt, D., Blasco, R., Pedroza-Islas, R., Hermida, L. G. (2013). Microencapsulation of linseed oil by spray drying for functional food application. Food Res. Int., 52(2), 473-482.
 
[20] Cortés-Rojas, D. F., Souza, C. R. F., Oliveira, W. P. (2014). Encapsulation of eugenol rich clove extract in solid lipid carriers. J. Food Eng., 127:34–42.
 
[21] Piacentini, E., Giorno, L., Dragosavac, M. M., Vladisavljević, G. T., and Holdich, R.G. (2013). Microencapsulation of oil droplets using cold water fish gelatine/gum arabic complex coacervation by membrane emulsification. Food Res. Int., 53 (1), 362–372.
 
[22] Versic, R. J. (2003). Coacervation for flavor encapsulation. J. Microencapsul., 14,126-131.
 
[23] Dong, Z. J., Touré, A., Jia, C. S., Zhang, X. M.,  Xu, S. Y. (2007). Effect of processing parameters on the formation of spherical multinuclear microcapsules encapsulating peppermint oil by coacervation. J. Microencapsul., 24, 634-46.
 
[24] Dong, Z. J., Xia, S. Q., Hua, S., Hayat, K., Zhang, X. M., Xu, S. Y. (2008). Optimization of cross-linking parameters during production of transglutaminase-hardened spherical multinuclear microcapsules by complex coacervation. Colloids Surf. B., 63(1), 41-47.
 
[25] Drusch, S., Berg, S. (2008). Extractable oil in microcapsules prepared by spray-drying: localisation, determination and impact on oxidation stability. Food Chem., 109, 17–24.
 
[26] Alvim, I.D., Grosso, C.R.F. (2010). Microparticles obtained by complex coacervation: in­uence of the type of reticulation and the drying process on the release of the core material. Ciênc. Tecnol. Aliment Campinas, 30(4): 1069-1076.
 
[27] Carneiro, H.C.F., Tonon, R.V., Grosso, C.R.F., Hubinger, M.D. (2013).  Encapsulation efficiency and oxidative stability of flaxseed oil microencapsulated by spray drying using different combinations of wall materials. J. Food Eng., 115, 443–45.
 
[28] Gan, C.Y., Cheng, L.H., Easa, A.M. (2008). Evaluation of microbial transglutaminase  ribose crosslinked soy protein isolate-based microcapsules containing fish oil. Innov. Food Sci. Emerg. Technol., 9(4), 563–9.
 
[29] Kirk, R. S., Sawyer, R. (1991). Pearson’s Composition and Analysis of Foods (9th ed.). London: Longman Scientific  Technical. (pp. 28–31).
 
[30] O’Connor, C. J., Lal, S. N. D., Eyres, L. (2007). Handbook of Australasian edible oils. Auckland, New Zealand: Oils and Fats Specialist Group of NZIC.
 
 [31] Pourashouri, p., Shabanpour, B., Razavi, S. H., Jafari, S. M., Shabani, A., Aubourg, S. (2014). Oxidative stability of spray-dried microencapsulated fish oils with different wall materials. J. Aquat. Food Prod. T, 23,567–578.
 
 [32] Bao, S. S., Hu, X. C., Zhang, K., Xu, X. K., Zhang, H. M.,  Huang. (2011). Characterization Of Spray-Dried Microalgal Oil Encapsulated In Cross-Linked Sodium Caseinate Matrix Induced By Microbial Transglutaminase. J. Food Sci., 76(1), 112-118.
 
[33] Yang, Z., Peng, Z., Li, J., Li, S., Kong, L., Li, P. (2014). Development and evaluation of novel flavour microcapsules containing vanilla oil using complex. J. Food Process. Preserv., 33(2):255–270.
 
[34] Santos, M. G., Bozza, F., Thomazini,T.,  M. Favaro-Trindade. C. S. (2015). Microencapsulation of xylitol by double emulsion followed by complex Coacervation. Food Chem., 171, 32–39.
 
[35] Alvim, I. D., Grosso, C. R. F. (2010). Microparticles obtained by complex coacervation: Influence of the type of reticulation and the drying process on the release of the core material. Ciênc. Tecnol. Aliment., 30, 1069–1076.
 
[36] Matalanis, A., Jones, O. G.,  McClements, D. J. (2011). Structured biopolymer-based delivery systems for encapsulation, protection, and release of lipophilic compounds. Food Hydrocoll., 25, 1865–1880.
 
[37] Lakkis, J. M. (2007). Frontmatter, in Encapsulation and Controlled Release Technologies in Food Systems, Blackwell Publishing, Ames, Iowa, USA. doi: 10.1002/9780470277881.fmatter
 
[38] LeeS.J.Rosenberg, M. (2000). Whey protein-based microcapsules prepared by double emulsification and heat gelation. Food Sci. Technol., 33(2), 80-88.
 
[39] Liu, S. N., Low, H., Nickerson, M. T. (2010). Entrapment of flaxseed oil within gelatin-gum arabic capsules. J. Am. Oil Chem. Soc., 87, 809-815.
 
[40] Moreau, D. L., Rosenberg, M. (1998). Porosity of whey protein-based microcapsules containing anhydrous milkfat measured by gas displacement phenometry. J. Food Sci., 63, 819–23.
 
[41] Sun-Waterhouse, D., Zhou, J., Miskelly, G. M., Wibisono, R., Wadhwa, S. S. (2011). Stability of encapsulated olive oil in the presence of caffeic acid. Food Chem., 126(3), 1049–1056.
 
 [42] Drusch., S. (2007). Sugar beet pectin: a novel emulsifying wall component for microencapsulation of lipophilic food ingredients by spray-drying. Food Hydrocoll., 21, 1223–1228.
 
 [43] Baik, M., Suhendro, E., Nawar, W., Mcclements, J., Decker, E., Chinachoti, D. (2004). Effects of antioxidants and humidity on the oxidative stability of microencapsulated fish oil. J. Am. Oil Chem. Soc., 81, 355–360.
 
[44] Drusch, S., Serfert, Y., Heuvel, A. V. D., Schwarz, K. (2006). Physicochemical characterization and oxidative stability of fish oil encapsulated in an amorphous matrix containing trehalose. Food Res. Int., 39, 807–815.
 
[45] Peng, Z., Li, J., Guan, Y., Zhao, G. (2013). Effect of carriers on physicochemical properties, antioxidant activities and biological components of spray-dried purple sweet potato flours. LWT – Food Sci. Technol. 51, 348–355.
 
[46] Imagi, J., Kako, N., Nakanishi, K., Matsuno, R. (1990). Entrapment of Liquid Lipids in Matrixes of Saccharides. J. Food Eng., 12, 207-222.
 
[47] Liu, S. N., Low, H., Nickerson, M. T. (2010). Entrapment of flaxseed oil within gelatin-gum arabic capsules. J. Am. Oil Chem. Soc., 87, 809-815.