نوع مقاله : مقاله پژوهشی
نویسندگان
گروه صنایع غذایی دانشگاه علوم کشاورزی و منابع طبیعی ساری
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Introduction: Oxidative rancidity is a major challenge for the shelf-life of polyunsaturated vegetable oils like sunflower oil.s. Barley and rice sprouts are rich sources of bioactive phenolic and flavonoid compounds with high antioxidant potential.. Cold plasma (CP) and ultrasound-assisted extraction (UAE) are two promising green methods. CP modifies surface structure via active species, while UAE enhances mass transfer through cavitation. Their combination may have synergistic effects. Furthermore, the low stability and bioavailability of plant extracts in food matrices necessitate encapsulation. Nanocapsulation using natural biopolymers like whey protein isolate (WPI) and Lepidium sativum seed gum (LPSG) can protect bioactive compounds and enable controlled release. This study aims to: 1) Evaluate and compare the efficiency of UAE, CP, and combined CP-UAE methods for extracting bioactive compounds from barley and rice sprouts, 2) Investigate the impact of encapsulating the best-performing extracts in WPI and LPSG on the physicochemical properties of nanocapsules, and 3) Determine the efficacy of optimized nanocapsules in enhancing the oxidative stability of sunflower oil, presenting an integrated natural alternative to synthetic antioxidants.
Materials and Methods: Barley and rice sprouts were germinated,dried, and powdered. Extracts were obtained using three methods: UAE (35 kHz, 35°C, 30 min), CP (Dielectric Barrier Discharge, nitrogen, 30 kV, 5 min), and a combined method (CP pre-treatment followed by UAE). Extraction yield, total phenolic content (TPC, Folin-Ciocalteu), and total flavonoid content (TFC, aluminum chloride method) were measured. Antioxidant activity was evaluated via DPPH radical scavenging and FRAP assays at concentrations of 200-1000 ppm. Selected extracts were nanocapsulated using an emulsification-sonication method with WPI and LPSG as wall materials, followed by freeze-drying.
Results and Discussion: The combined CP-UAE method yielded the highest extraction efficiency,TPC, and TFC for both sprouts, with barley sprout extract (BCP-UAE) showing the best results (14.01% yield, 4.11 mg GAE/g, 3.65 mg QE/g).
کلیدواژهها [English]
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