Isolation and identification of a strain producing the phospholipase enzyme from waste oil industry and its mutant

Document Type : Research Article


1 PhD Student, Food Science and Technology, Tarbiat Modarres University

2 Associate Professor, Food Science and Technology, Tarbiat Modarres University

3 Professor, Food Science and Technology, Tarbiat Modarres University

4 Associate Professor, Shahid Beheshti University


In the present study, among several species capable of producing phospholipase were isolated from the oil industry wastewater, Tricoderma atroviride ZB-ZH 192 identified and selected for further investigation. In order to increase phospholipase activity, the selected strain was exposed to random mutation by gamma rays irradiation at various doses of 200, 400, 600 and 800 Gy. Comparison between wild and mutant-types of enzymes showed that gamma radiation of 400 Gy led to stable mutation with increasing phospholipase activity rate from  1.96 l to 3.71 (U/mL) and biomass rate of production from 8.13 to 13.17 (g/L). In order to screen and evaluate factors affecting the enzyme activity and biomass production by selected mutant, Plackett-Burman design with seven variables each at two levels including temperature, time, amount of soybean phospholipids (as a carbon source), peptone level (as nitrogen source), equal ratio of mono and di-potassium hydrogen phosphate (as phosphorus source), seed size and seed age were studied using JMP software. The results showed that high levels of time, temperature and soybean phospholipids as a carbon source had significant effect on the enzyme activity, while factors such as amount of peptone as a source of nitrogen and time had significant effect on the biomass production.


Main Subjects

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