الکتروریزپوشانی لیکوپن در ساختار میکروالیاف پروتئینی: بررسی خصوصیات فیزیکوشیمیایی و زیست‌دسترسی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجو کارشناسی ارشد گروه علوم و مهندسی صنایع غذایی دانشگاه فردوسی مشهد

2 استاد، گروه علوم و مهندسی صنایع غذایی، دانشگاه فردوسی مشهد

3 استادیار، گروه نانوفناوری مواد غذایی،موسسه پژوهشی علوم و صنایع غذایی، مشهد

چکیده

لیکوپن رنگ‌دانه کاروتنوئیدی چربی‌دوستی است که دارای خواص سلامتی بخش ویژه‌ای می‌باشد، به این دلیل بسیاری از محققین، آن را به ‌عنوان یک ماده ارزشمند می‌شناسند. پایداری بسیاری از ترکیبات زیست فعال به علت فرآیندهای گوناگون فیزیکوشیمیایی و فیزیولوژی محدود می‌شود، به همین منظور استفاده از روش‌های مختلف ریزپوشانی جهت آزاد شدن کنترل‌شده و افزایش میزان پایداری ترکیبات زیست فعال، امری ضروری می‌باشد. در این پژوهش تأثیر غلظت‌های مختلف محلول زئین (35 و 30، 25، 20، 15 درصد وزنی/حجمی) بر تولید الیاف زئین با استفاده از روش الکتروریسی مورد بررسی قرار گرفت. در ادامه از غلظت بهینه جهت ریزپوشانی لیکوپن در دو سطح (0.075، 0.05درصد وزنی/ وزنی) استفاده شده است. خصوصیات فیزیکی و شیمیایی الیاف زئین حاوی لیکوپن مورد بررسی قرار گرفت. همچنین پروفایل رهایش لیکوپن در سه فاز دستگاه گوارش انسان (دهان، معده و روده کوچک) بررسی شد. تصاویر حاصل از میکروسکوپ الکترونی روبشی (SEM) نشان داد الیاف یکنواخت، همگن و بدون مهره برای زئین حاصل گردیده است. راندمان ریزپوشانی لیکوپن در دو نمونه بین 88.07-85.68درصد متغیر بوده است. نتایج حاصل آزمون FTIR حاکی از آن است که ریزپوشانی به‌صورت موفقیت‌آمیز صورت گرفته و از نوع فیزیکی می‌باشد. علاوه بر این رهایش کنترل‌شده و پایدار از لیکوپن در دستگاه گوارش مشاهده‌ شده است که این سبب افزایش در زیست‌دسترسی آن شده است. ریزپوشانی با استفاده از الیاف الکتروریسی شده زئین پتانسیل بالایی به ‌عنوان یک سیستم رسانش هدفمند مناسب برای لیکوپن دارد. از این‌رو استفاده از این روش برای ریزپوشانی لیکوپن در صنعت غذا توصیه می‌شود.

چکیده تصویری

الکتروریزپوشانی لیکوپن در ساختار میکروالیاف پروتئینی: بررسی خصوصیات فیزیکوشیمیایی و زیست‌دسترسی

تازه های تحقیق

  • شرایط بهینه فرایند الکتروریسی جهت پوشش دهی لیکوپن درون میکروالیاف زئین تعیین شد.
  • 2. اثر محیط دستگاه گوارش بر روی لیکوپن درون پوشانی شده بررسی شد.
  • 3- الکتروریزپوشانی لیکوپن در میکروالیاف زئین سبب افزایش زیست دسترسی آن می‌شود.
  • 4- به دام افتادن لیکوپن درون الیاف پروتئینی به صورت فیزیکی روی می‌دهد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Electro-Encapsulation of Lycopene in Protein Microfiber Structure: Physicochemical and Bioaccessibility Characteristics

نویسندگان [English]

  • Esmaeel Charpashlo 1
  • Mohebbat Mohebbi 2
  • Behrouz Ghorani 3
1 M.Sc. student, Department of Food Science and Engineering, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
2 Professor, Department of Food Science and Engineering, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
3 Assistant Professor, Department of Food Nanotechnology, Research Institute of Food Science and Technology (RIFST), Mashhad, Iran
چکیده [English]

Lycopene is a carotenoid pigment withsome special health attributes, which have attracted many researchers’ attention as a valuable ingredient. The stability of many bioactive compounds is limited due to various physicochemical and physiological processes. For this purpose, the application of different encapsulation methods for controlled release and improved stability of bioactive compounds is of great importance. In this study, the effect of various solution concentrations (15, 20, 25, 30 and 35 %w/v) on the production of zein fibers was studied. The optimized concentration was then, applied for the encapsulation process of lycopene at two levels (0.05, 0.075 %w/w). The physical and chemical properties of lycopene-containing zein fibers and the profile of lycopene release in three phases of gastrointestinal (GI) tract (mouth, stomach and small intestine) were investigated. The results of scanning electron microscopy (SEM) showed that uniform, homogeneous and beads-free fibers were obtained at the optimum conditions. The lycopene loading efficiency was measured between 85.68-88.07%. The results of FTIR test indicate that the physical entrapment of lycopene in zein microfibers was successfully occurred. In addition, controlled and stable release of lycopene in the GI simulated system which shows its appropriate bioaccessibility was observed. Encapsulation using zein electrospun microfiber has the potential to serve as a targeted delivery system for lycopene. The use of this method is therefore, recommended for the encapsulation of lycopene in the food industry.

کلیدواژه‌ها [English]

  • Electro-Encapsulation
  • Electrospinning
  • Fiber
  • Bioaccessibility
  • Lycopene
  • Zein
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