حامل‌های لیپیدی نانوساختار(NLC) حاوی ویتامینA پالمیتات؛ عوامل موثر بر اندازۀ ذرات، کارایی درون‌پوشانی و پایداری

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکترا، دانشگاه تبریز

2 دانشیار، دانشگاه تبریز

3 دانشیار، دانشگاه علوم پزشکی تبریز

4 استاد، دانشگاه تبریز

5 دانش آموخته، دانشگاه تبریز

چکیده

درون‌پوشانی ترکیبات غذا- دارو مانند ویتامین های محلول در چربی، توسط حامل‌های لیپیدی نانوساختار(NLC)، روشی بالقوه مؤثر در افزایش پایداری آنها، در طی فرایند و نگه‌داری می‌تواند باشد. در این پژوهش، NLC حامل ویتامین A با استفاده از روش هوموژناسیون گرم تولید و فرمولاسیون بهینه این نانوحامل‌های کلوئیدی با تغییر شرایط مختلف از جمله نوع فاز لیپیدی جامد (کامپریتول888ATO، ستیل پالمیتات، پیرسرول AT05)، نوع فاز روغنی حامل (میگلیول 812 و اکتیل اکتانیت)، نسبت لیپید جامد به روغن مایع و غلظت سورفکتانت مشخص شد. آزمون‌های تعیین اندازۀ ذرات، کارایی درون پوشانی، بررسی ریزساختار نانوحامل و پایداری درون‌پوشانی نانوحامل‌ها جهت تعیین خواص کاربردی نمونه‌ها انجام گرفت. نوع لیپید جامد و نسبت لیپید جامد به مایع اثر چشمگیری در اندازة ذرات NLC داشتند و غلظت سورفاکتانت به جز در مقادیر بالا (8٪) تأثیر قابل ملاحظه‌ای را نشان نداد. در حالیکه، افزایش غلظت سورفاکتانت موجب افزایش کارایی و پایداری درون‌پوشانی گردید. فرمولاسیون حاوی 6% (w/v) پلوکسامر 407 و نسبت 10:1 پیرسرول(فاز لیپیدی) به فاز روغنی اکتیل اکتانیت (حاوی 3% ویتامین A پالمیتات)، به عنوان فرمولاسیون بهینه با کمترین اندازه ذرات (78 نانومتر با ضریب پراکنش 602/0) و با کارایی درون پوشانی %5/98 تعیین شد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Vitamin A Palimitae Bearing Nano-structured Lipid Carrier (NLC): Factors Affecting Particle Size, Encapsulation Efficiency and Stability

نویسندگان [English]

  • Akram Pezeshky 1
  • Babak Ghanbarzadeh 2
  • Hamed Hamishehkar 3
  • Mohammad Moghadam 4
  • Maryam Mohammadi 5
1 Department of Science and Technology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.
2 Department of Food Science and Technology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
3 Department of Pharmaceutical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Department of Crop Production and Plant breeding, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
5 Department of Science and Technology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
چکیده [English]

The encapsulation of nutraceutical compounds in lipid-based carrier systems, ­like nano structured lipid carrier (NLC), is effective in preservation against their degradation during processing and storage. The objective of the present work was to prepare vitamin A palmitate NLC and investigate the influence of several factors on particle size, encapsulation efficiency and stability of prepared particles.  The hot homogenization technique was applied to produce NLC dispersions and the optimum formulation determined with different circumstances changes, including surfactant concentration, solid lipid type (Compritol ATO 888, Cetyl palmitate, Precirol ATO 5), liquid oil type (Miglyol 812, Octyl octanoate) and  lipid-oil ratio. NLCs prepared at 6% poloxamer 407 concentration and at 10:1  precirol (solid lipid) to Octyloctanoate containing 3% vitamin A palmitate concentration (liquid oil) ratio showed significantly smaller size and narrower size distribution range (78 nm and 0.6 respectively). The encapsulation efficiency of this optimum formulation was 98.5% and was stable during 70 days storage. The solid lipid type and lipid-oil ratio had significant influence on particle size of formulations and the surfactant concentration showed no significant effect except at higher concentration like 8%.

کلیدواژه‌ها [English]

  • Nanostructured lipid carrier
  • Vitamin A palmitate
  • Encapsulation
  • stability
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