بهینه‌سازی و بررسی مقایسه‌ای محتوای فنولی ترکیبات زیست فعال استخراج‌شده از برگ گیاه فیجوا (Feijoa sellowiana) به کمک امواج فراصوت

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه زنجان

2 استادیار، گروه علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه زنجان

چکیده

در این پژوهش به‌منظور بررسی، مدل‌سازی و بهینه‌سازی اثر متغیرهای مستقل فرایند استخراج با حلال به کمک امواج فراصوت شامل شدت امواج فراصوت ( W/cm200/714، 50/1428 و 00/2143)، چرخه کاری (٪50- 75-100 درصد) و دمای استخراج (Cº 30-45-60) بر محتوای فنولی ترکیبات زیست فعال استخراج‌شده از برگ گیاه فیجوا از روش‌شناسی سطح پاسخ بر اساس طرح باکس- بنکن استفاده شد. میزان کل ترکیبات فنولی توسط روش فولین - سیوکالتیو اندازه‌گیری شد. با استفاده از تجزیه‌وتحلیل رگرسیون خطی چندگانه، مدل چندجمله‌ای درجه دوم کامل برای میزان کل ترکیبات فنولی به دست آمد. با استفاده از تکنیک بهینه‌سازی عددی شرایط بهینه به‌صورت W/cm2 34/1749، ٪39/86 و C º 49/32 تعیین شد. فعالیت ضد رادیکالی ترکیبات فنولی به‌دست‌آمده تحت شرایط بهینه با روشهای دی‌فنیل پیکریل هیدرازیل (DPPH) و 2و ´2 آزینوبیس (3 - متیل بنزو تیازولین - 6 - سولفونیک اسید) (ABTS) اندازه‌گیری شد. عصاره ترکیبات زیست‌فعال دارای میزان کل ترکیبات فنولی mg GAE/g 12/0 ± 20/381 و فعالیت ضد رادیکالی ٪10/0 ± 43/81 مهار رادیکال‌های آزاد DPPH و ٪26/0 ± 63/86 مهار رادیکال‌های آزاد ABTS بود. مدل ارائه‌شده برای پیش‌بینی مقدار پاسخ نتیجه بسیار نزدیکی با داده‌های تجربی داشت. تکنیک استخراج با حلال متلاطم جهت بررسی مقایسه‌ای موردمطالعه قرار گرفت که در این تکنیک تأثیر متغیر شدت دور در دامنه rpm50-300 بر میزان کل ترکیبات فنولی مطالعه شد. بر اساس نتایج به‌دست‌آمده، با استفاده از تکنیک حلال متلاطم بالاترین میزان کل ترکیبات فنولی (mg GAE/g 12/0 ± 95/352) درشدت دور rpm 200 به دست آمد. ترکیبات به‌دست‌آمده دارای فعالیت ضد رادیکالی ٪16/0 ± 33/73 مهار رادیکال‌های آزاد DPPH و ٪12/0 ± 20/82 مهار رادیکال‌های آزاد ABTS بودند. با توجه به نتایج بررسی مقایسه‌ای می توان بیان نمود که با استفاده از امواج فراصوت امکان دستیابی به ترکیبات زیست فعال فنولی بیشتر و فعالیت ضد رادیکالی بالاتر در مدت‌زمان کوتاه‌تری وجود دارد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Optimization and comparative evaluation of ultrasound-assisted extraction of bioactive phenolic compounds from feijoa (Feijoa sellowiana) leaves

نویسندگان [English]

  • Yasaman Poodi 1
  • Mandana Bimakr 2
  • Ali Ganjloo 2
  • Soheila Zarringhalami 2
1 Department of Food Science and Technology, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
2 Assistant Professor, Department of Food Science and Technology, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
چکیده [English]

In this study, the effect of ultrasound-assisted extraction (UAE) process independent variables including ultrasonic intensity (714.00, 1425.50 and 2143.00 w cm-2), duty cycle (50, 75 and 100%) and extraction temperature (30, 45 and 60 °C) on feijoa leaves bioactive phenolic compounds was investigated and optimized using response surface methodology based on Box-Behnken design. The total phenolic content of extracts was measured using Folin- Ciocalteu method. Quadratic polynomial model was obtained for total phenolic compounds using multiple linear regression analysis. The optimal conditions were determined as 1749.34 W cm-2 ultrasonic intensity, 86.39% duty cycle and 32.49 °C extraction temperature. The radical scavenging activity of phenolic compounds obtained under optimized UAE conditions was assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2´-Azinobis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) radical scavenging activity methods. Based on results obtained, the total phenolic compound and radical scavenging activity of extract obtained under optimal conditions were 381.20 ± 0.12 mg GAE/g, 81.43 ± 0.10% of DPPH radical inhibition and 86.63 ± 0.26% of ABTS radical inhibition, respectively. The model obtained for prediction of dependent variable has a good closeness with experimental results. The agitated bed extraction technique was investigated to carry out the comparative evaluation. The effect of agitation intensity (50-300 rpm) on total phenolic content of extracts obtained using this technique was studied. Based on results obtained, the highest value of total phenolic content (352.95 ± 0.12 mg GAE/g) could be achieved using 200 rpm agitation intensity. The radical scavenging activity of extract was 73.33 ± 0.16% of DPPH radical inhibition and 82.20 ± 0.12% of ABTS radical inhibition. According to the results obtained it could be stated that ultrasound-assisted extraction is a promising technique to obtain extracts contain more phenolic compounds and higher radical scavenging activity during short extraction time.

کلیدواژه‌ها [English]

  • Feijoa
  • Ultrasound-assisted extraction
  • Response surface methodology
  • Total phenolic compounds
  • Radical scavenging activity
  • Agitated bed extraction technique
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