پیش‌بینی فعالیت آنزیم پراکسیداز با استفاده از تصویربرداری فراطیفی فروسرخ نزدیک در سیب رددلیشز طی دوره نگهداری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشیار، گروه مهندسی بیوسیستم، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران

2 دانش آموخته دکتری، گروه مهندسی بیوسیستم، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران

چکیده

از آنجا که فعالیت آنزیمی یکی از پارامترهای کیفی مهم سیب به شمار می رود، در این تحقیق اثر طول دوره انبارداری سرد بر مقدار فعالیت آنزیم پراکسیداز در سیب رقم رددلیشز به مدت 60 روز مطالعه شد. تصویربرداری فراطیفی بازتابی در محدوده طول‌موج nm 1100-400 انجام و فعالیت آنزیمی در نمونه‌ها نیز براساس روش های استاندارد اندازه گیری شد. پس از حذف نویزها با آنالیز PCA، برای بهبود طیف، پیش‌پردازش‌های اولیه مختلف اعمال و اثرات آن‌ها مورد مطالعه قرار گرفت. مدل مناسب با استفاده از روش حداقل مربعات جزئی (PLS) تعیین شد. طول‌موج‌های مؤثر با استفاده از الگوریتم های پیش بینی متوالی (SPA) و ضریب رگرسیون (RC) بهترین مدل انتخاب و با استفاده از روش‌های مختلف مدل‌سازی شد. بر اساس آنالیز PLS بهترین نتایج با پیش‌پردازش هموارسازی ساویتزکی-گولای با 574/0=RMSEC، 948/0=R2c، 518/0=RMSECV، 940/0=R2CV حاصل شد. بر اساس آنالیز داده های پیش پردازشی با ضریب رگرسیون (RC) و الگوریتم های پیش بینی متوالی (SPA) 9 طول‌موج به عنوان طول‌موج های مؤثر در تخمین فعالیت آنزیم پراکسیداز در نمونه ها تعیین شدند. در مدل‌سازی با استفاده از طول‌موج های مؤثر، مدل تلفیق شبکه عصبی مصنوعی (ANN) و الگوریتم های پیش بینی متوالی (SPA) بهترین نتیجه را داشت. در نتیجه به نظر می‌رسد روش تصویربرداری فراطیفی می تواند به عنوان ابزاری با ارزش برای پیش بینی فعالیت آنزیم پراکسیداز در سیب طی دوره نگه داری بکار برده شود و طول‌موج انتخابی می تواند منابع بالقوه برای توسعه یک ابزار غیرمخرب باشد.

چکیده تصویری

پیش‌بینی فعالیت آنزیم پراکسیداز با استفاده از تصویربرداری فراطیفی فروسرخ نزدیک در سیب رددلیشز طی دوره نگهداری

تازه های تحقیق

  • از تصویربرداری فراطیفی مادون قرمز نزدیک برای تخمین فعالیت آنزیم پرکسیداز در سیب رقم رد دلیشز طی دوره نگه­داری استفاده شد.
  • اثر روش­های مختلف پیش پردازش بر روی مدل PLS مورد مطالعه قرار گرفت.
  • طول موج های موثر برای تشخیص pH سیب بر اساس ضریب رگرسیون بهترین مدل PLS و الگریتم پیش­بینی متوالی (SPA) انتخاب شدند.
  • مدل­های مختلف رگرسیونی با طول موج­های موثر ایجاد و کارآیی آن­ها با یکدیگر مقایسه شد

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Prediction of peroxidase activity using near infrared hyperspectral imaging in red delicious apple fruit during storage time

نویسندگان [English]

  • Abdollah Golmohammadi 1
  • Mahsa Sadat Razavi 2
  • Mohammad Tahmasebi 2
1 Associate Professor, Department of Biosystems Engineering, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
2 PhD Graduated, Department of Biosystems Engineering, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
چکیده [English]

Regarding this fact that peroxidase (POD) activity is considered as one of the important qualitative parameters of apple fruits, in this study, the effect of cold storage on POD activity of Red Delicious apples were investigated during 60 days. Hyperspectral reflecting imaging in range of 400-1000 nm has been applied while POD of samples were measured according to standard methods. After discarding noises using principal component analysis (PCA), to improve spectrum, different primary pre-processing had been applied and their effects were investigated. The suitable model was obtained via Partial Least Square method (PLS). Important wavelengths were selected based on regression coefficient of the best model includes large absolute values of weighted regression coefficients (RC) and sequential predictions algorithm (SPA) and using various techniques were modeled. Concerning the PLS analysis, the best results were obtained through smoothing Savitzky-Golay pre-processing with mean square root error (RMSE) of 0.475 and 0.518 and coefficient of determination (R2) of 0.948 and 0.940 for calibration and validation data, respectively. According to RC and SPA, 9 wavelengths were determined as the best. In modeling by efficient wavelength, artificial neural network (ANN) and SPA Combined Model gave the best result. The results indicated that hyperspectral imaging could be considered as a valuable tool for POD activity prediction and the selected wavelengths could be potential resources for instrument development.

کلیدواژه‌ها [English]

  • Shelf life
  • peroxidase
  • hyperspectral imaging
  • apple
  • non-destructive
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