Production of Functional Fermented Milk by Lactobacilli Isolated from Traditional Iranian Dairy Products

Document Type : Research Article

Authors

1 Ph.D student, Department of Biotechnology, Iranian Research Organization for Science & Technology (IROST), Tehran, Iran

2 Professor, Department of Biotechnology, Iranian Research Organization for Science & Technology (IROST), Tehran, Iran

3 Assistant Professor, Department of Food Science and Technology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran

4 Assistant Professor, Department of Biotechnology, Iranian Research Organization for Science & Technology (IROST), Tehran, Iran.

Abstract

Lactic acid bacteria have a long history in the production of fermented dairy products. They are of great importance in the production of bioactive peptides and increasing the health effects due to their proteolytic ability. In the present study, the ability of two strains of Lactic acid bacteria were investigated after isolation from Iranian dairy products and were compared in terms of fermentation ability, proteolytic properties, and production of fermented milk with antioxidant and acceptable sensory properties.
Two isolated strains, including Lactobacillus fermentum (PTCC 1929) and Lactobacillus helveticus (PTCC1930)  were identified based on the ability of sugar fermentation and 16S rRNA sequence analysis. The results showed that in spite of the better growth of Lactobacillus fermentumbacteria in the first 24 hours, the initial acidification was higher in L. helveticus.  The pH value in the Lactobacillus helveticussample was less than 4 in the early 24 hours, while this value was achieved after 72 hours in L. fermentum. In the co-culture, the pH reduction steps reached at a minimum level of 3.63 with the initial and secondary acidifications of Lactobacillus helveticusand L. fermentum, respectively. The results showed that, despite the higher proteolysis ability of Lactobacillus helveticus, the antioxidant activity of the sample containing Lactobacillus fermentumwas 1.5 to 1.7 times higher. The highest antioxidant activity was observed in all samples at the first 24 hours. Increase in proteolysis did not cause an increase in the production of antioxidant peptides. Milk fermented by Lactobacillus fermentum for 24 hours was significantly lower in taste and acceptability score compared to the other samples (P<0.05). In general, the results showed that two strains co-culture has a better ability to produce antioxidant bioactive peptides with a suitable overall acceptance and has the potential for the production of fermented dairy products with healthy properties.

Graphical Abstract

Production of Functional Fermented Milk by Lactobacilli Isolated from Traditional Iranian Dairy Products

Highlights

  • L.fermentum and L.helveticus had the ability to decrease the pH in fermented milk respectively, by 24 and 72 hours.
  • Despite the higher proteolysis ability of L. helveticus, the antioxidant activity of the sample containing L. fermentum was 1.5 to 1.7 times higher.
  • The highest antioxidant activity was observed in all samples at the first 24 hours. Increase in proteolysis did not cause an increase in the production of antioxidant peptides.
  • Despite the lower flavor and total acceptance scores of samples fermented for 24 hours by L.fermentum, no significant differences was observed between other samples.

Keywords

Main Subjects

References

[1]   Virtanen, T., Pihlanto, A., Akkanen, S. Korhonen, H. ( 2007). Development of antioxidant activity in milk whey during fermentation with lactic acid bacteria. J.Appl.Microbiol., 102(1), 106-115.
[2]     ]2[Hafeez, Z., Cakir-Kiefer, C., Roux, E., Perrin, C., Miclo, L. Dary-Mourot, A. (2014). Strategies of producing bioactive peptides from milk proteins to functionalize fermented milk products. Food Res. Int., 63, 71-80.
[3]     ]3[Benkerroum, N. (2010). Antimicrobial peptides generated from milk proteins: a survey and prospects for application in the food industry. A review. Int. J. Dairy Technol., 63(3), 320-338.
[4]     ]4[Agyei, D. Danquah, M.K. (2011). Industrial-scale manufacturing of pharmaceutical-grade bioactive peptides. Biotechnol.Adv., 29(3), 272-277
[5]     ]5[Mirzaei, M., Mirdamadi, S., Ehsani, M.R., Aminlari, M. Hosseini, E. ( 2015). Purification and identification of antioxidant and ACE-inhibitory peptide from Saccharomyces cerevisiae protein hydrolysate. J. Funct. Foods, 19, 259-268.
[6]     ]6[Aspri, M., Leni, G., Galaverna, G. , Papademas, P. ( 2018). Bioactive properties of fermented donkey milk, before and after in vitro simulated gastrointestinal digestion. Food Chem., 268, 476-484.
[7]     ]7[Jafari, M., Jahed Khaniki ,G., Roshanzamir, M., Sadighara, P.(2017 ). Antioxidant activity of raw milk and dairy products commonly consumed in Fars province,Iran.J. Food. Safe  Hyg.,3(1-2),21-6.
[8]     ]8[Huma, N., Rafiq, S., Sameen, A., Pasha, I. Khan, M.I. ) 2018(. Antioxidant potential of buffalo and cow milk Cheddar cheeses to tackle human colon adenocarcinoma (Caco-2) cells. Asian. Austral. J. Anim., 31(2), 287.
[9]     ]9[Aloğlu, H.Ş., Öner, Z. ) 2011(. Determination of antioxidant activity of bioactive peptide fractions obtained from yogurt. J. Dairy Sci., 94(11), 5305-5314.
[20] ]10[Conway, V., Gauthier, S.F.,  Pouliot, Y. )2012(. Antioxidant activities of buttermilk proteins, whey proteins, and their enzymatic hydrolysates. J. Agric. Food Chem., 61(2), 364-372.
[11] ]11[De Lima, M.D.S.F., da Silva, R.A., da Silva, M.F., da Silva, P.A.B., Costa, R.M.P.B., Teixeira, J.A.C., Porto, A.L.F., Cavalcanti, M.T.H. (2018). Brazilian Kefir-fermented sheep’s milk, a source of antimicrobial and antioxidant Peptides. Probiotics. Antimicrob Proteins., 10(3),  446-455.
[12] ]12[Soleymanzadeh, N., Mirdamadi, S., Kianirad, M. (2016). Antioxidant activity of camel and bovine milk fermented by lactic acid bacteria isolated from traditional fermented camel milk (Chal). Dairy. Sci. Technol., 96(4),443-457.
[13]                       ]13[Rahmawati, I., Suntornsuk, W. (2016). Effects of fermentation and storage on bioactive activities in milks and yoghurts.Procedia Chem.,18, 53-62
[14] ]14[Namdari,A., Nejati,F. ( 2016). Development of antioxidant activity during milk fermentation by wild isolates of Lactobacillus helveticus. Appl. Food Biotechnol.,3(3),178-86.
[15] ]15[Ayyash, M., Al-Dhaheri A.S., Mahadin, S. Al., Kizhakkayil, J., Abushelaibi, A. ( 2018 ). In vitro investigation
[16] of anticancer, antihypertensive, antidiabetic, and antioxidant activities of camel milk fermented with camel milk probiotic: A comparative study with fermented bovine milk.J. Dairy. Sci.,101(2),900-11.
[17] ]16[Sneath, P.H., Mair, N.S., Sharpe, M.E. and Holt, J.G. ( 1986). Bergey's manual of systematic bacteriology. Volume 2. Williams & Wilkins.
[18] ] 17[Pailin, T., Kang, D.H., Schmidt, K. , Fung, D.Y.C. (2001). Detection of extracellular bound proteinase in Eps producing lactic acid bacteria cultures on skim milk agar. Lett. Appl. Microbiol., 33(1), 45-49.
[19] ]18[Oh, S., Kim, S.H., Worobo, R.W. (2000). Characterization and purification of a bacteriocin produced by a potential probiotic culture, Lactobacillus acidophilus 30SC. J. Dairy Sci., 83(12), 2747-2752.
[20] ]19[AOAC. (2002).Official Methods of Analysis. USA: Arlington,VA.
[21] ]20[Lowry, O.H., Rosebrough, N.J., Farr, A.L. , Randall, R.J. )1951(. Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193, 265-275.
[22] ]21[Church, F.C., Swaisgood, H.E., Porter, D.H. and Catignani, G.L. )1983(. Spectrophotometric assay using o-phthaldialdehyde for determination of proteolysis in milk and isolated milk proteins. J. Dairy Sci., 66(6), 1219-1227.
[23] ]22[Son, S. ,Lewis, B.A.( 2002). Free radical scavenging and antioxidative activity of caffeic acid amide and ester analogues: Structure− activity relationship. J.Agric.Food Chem., 50(3), 468-472.
[24] ]23[Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. )1999(. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radi.Biol.Med., 26(9-10),1231-1237.
[25] ]24[Zhang, S., Zhang, L., Jiao, Y., Luo, X., Li, H., Xin, L., Xue, C., Zhang, Y., Yi, H., Han, X. Ma, C.)  2014(. Technological characterization of lactic acid bacteria protease isolated from traditional chinese fermented milk. J. Food Qual., 37(6),395-402.
[26] ]25[Griffiths, M.W. , Tellez, A.M)  .2013(. Lactobacillus helveticus: the proteolytic system. Front. Microbiol., 4, 30.
[27] ]26[Taverniti, V. , Guglielmetti, S) .2012(. Health-promoting properties of Lactobacillus helveticus. Front. Microbiol., 3, 392.
[28] ]27[Moser, A., Berthoud, H., Eugster, E., Meile, L. , Irmler, S) . 2017(. Detection and enumeration of Lactobacillus helveticus in dairy products. Int. Dairy. J., 68, 52-59.
[29] ]28[Owusu-Kwarteng, J., Tano-Debrah, K., Akabanda, F. ,Jespersen, L.) 2015(. Technological properties and probiotic potential of Lactobacillus fermentum strains isolated from west african fermented millet dough. BMC Microbiol., 15(1), 261.
[30] ]29[Akabanda, F., Owusu-Kwarteng, J.,Tano-Debrah, K., Parkouda, C. and Jespersen, L.)  2014(. The use of lactic acid bacteria starter culture in the production of Nunu, a spontaneously fermented milk product in Ghana. Int. J. Food Sci., Article ID 721067.
[31] ]30[Dickson, E.M., Riggio, M.P. , Macpherson, L.) 2005(. A novel species-specific PCR assay for identifying Lactobacillus fermentum. J. Med. Microbiol., 54(3), 299-303.
[32] ]31[Savijoki, K., Ingmer, H. ,Varmanen, P. )2006(. Proteolytic systems of lactic acid bacteria. Appl. Microbiol. Biotechnol., 71(4), 394-406.
[33] ]32[Soleymanzadeh, N., Mirdamadi, S., Mirzaei, M., Kianirad ,M. ( 2019 ).Novel β-casein derived antioxidant and ACE-inhibitory active peptide from camel milk fermented by Leuconostoc lactis PTCC1899: Identification and molecular docking. Int. Dairy J. https://doi.org/10.1016/j.idairyj.2019.05.012.
[34] ]33[Tak, L., Bais, B., Singh, R. and Singh, S. ( 2018). Study of the anti-oxidant potential of buffalo milk using Lactobacillus helveticus and Lactobacillus fermentum. IJFFT., 8(1), 69-74.
[35] ]34[Wang, Y.C., Yu, R.C. ,Chou, C.C. )2006(. Antioxidative activities of soymilk fermented with lactic acid bacteria and bifidobacteria. Food Microbiol., 23(2), 128-135.
[36] ]35[Kim, S.K. ed. )2013(. Marine proteins and peptides: biological activities and applications. John Wiley & Sons., 393-397.
[37] ]36[Zhou, T., Huo, R., Kwok, L.Y., Li, C., Ma, Y., Mi, Z. , Chen, Y. )2019(. Effects of applying Lactobacillus helveticus H9 as adjunct starter culture in yogurt fermentation and storage. J. Dairy Sci. 02(1), 223-235.
Innovative Food Technologies
Volume 7, Issue 2
February 2020
Pages 243-255

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History

  • Receive Date: 28 May 2019
  • Revise Date: 10 July 2019
  • Accept Date: 02 September 2019

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