The Effect of Chitosan Type on Biodegradable Films from Gelatin Extracted from Huso Huso Skin Patches and Their Specifications and Characteristics

Document Type : Research Article

Authors

1 Ph. D student of Seafood Processing, Department of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

2 Associate Professor, Department of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

Abstract

Film forming capabilities of gelatin as well as chitosan have been used as edible films with suitable physical and mechanical properties in the packaging and food preserving industry. In this study, the effect of various chitosans (water-soluble, acid-soluble, oligosaccharide) was investigated with ratio of 50:50 chitosan-gelatin. The latter was extracted from farmed Huso huso skin. In order to evaluate the effect of chitosan kinds in combination with gelatin extracted from the fish, physical, mechanical experiments, and then optical and infrared spectroscopic tests, and thermal characteristics of the films were assessed. In this study, the highest tensile strength and elongation at break point was obtained in films containing 100% gelatin and 50-50% soluble chitosan and gelatin one, respectively. In the chitosan oligosaccharide film, the highest moisture and solubility was observed while in the pure gelatinous film the lowest amounts of them were portrayed. Regarding swelling rate results, all films (soluble in gelatin / chitosan) were dissolved, while the lowest percentage of swelling rate was found in the pure gelatinous film. The results of the water vapor permeability of the films showed that the gelatinous films combined with the acid-soluble and oligosaccharide chitosan showed the lowest and the highest permeability to water vapor, respectively. The optical properties and transparency of the films showed a significant difference. The results of the infrared spectroscopy indicated that the gelatin- chitosan oligosaccharide film due to the less breaking process and the stronger absorption of band in amide 1, demonstrated better properties. In addition, the thermal stability and the weighted remainder of the combined films were more than that of the pure gelatinous film, which can be attributed to the incorporation of chitosans in the film matrix.

Graphical Abstract

The Effect of Chitosan Type on Biodegradable Films from Gelatin Extracted from Huso Huso Skin Patches and Their Specifications and Characteristics

Highlights

  • Successfully utilization of beluga sturgeon (Huso huso) skin in order to extracting of gelatin capable of producing favorable film
  • Preparation of edible films of gelatin extracted from beluga sturgeon skin with good mechanical, water resistance and water vapor permeability properties.
  • Preparation of appropriate composite films of gelatin in combination with three different types of chitosan including water-soluble, acid-soluble and oligosaccharides of chitosan.

Keywords

Main Subjects

References

[1] Rhim, J., Ng, P. (2007). Natural biopolymer-based nanocomposite films for packaging applications. Crit. Rev. Food Sci. Nutr., 47(4), 411-43.
[2]Ketnawa, S., Benjakul, S., Martínez-Alvarez, O., Rawdkuen, S. (2017). Fish skin gelatin hydrolysates produced by visceral peptidase and bovine trypsin: Bioactivity and stability. Food Chem., 215, 383-390.
[3] Mahjoorian, A., Mortazavi, S. A., Tavakolipour, H., Motamedzadegan, A., Askari, B. (2013). Rheological properties of skin gelatin of Beluga Sturgeon (Huso huso) from The Caspian Sea. Eur. J. Biol. Res., 4(7), 227-23.
[4] Sánchez-González, L., Cháfer, M., Chiralt, A., González-Martínez, C.(2010).Physical properties of edible chitosan films containing bergamot essential oil and their inhibitory action on Penicillium italicum. Carbohyd polym., 82(2), 277-283.‏
[5]Aider, M., (2010), Chitosan application for active bio-based films production and potential in the food industry: Review, LWT-Food Sci. Technol., 43(6), 837-842.
[6] Abdollahi, M., Rezaei, M., Farzi, G.( 2012). A novel active bionanocomposite film incorporating rosemary essential oil and nanoclay into chitosan. J. Food Eng., 111(2), 343-350.‏
[7] Ojagh, S. M., Rezaei, M., Razavi, S. H. Hosseini, S. M. H., (2010), Development and evaluation of a novel biodegradable film made from chitosan and cinnamon essential oil with low affinity toward water, Food Chem.,122(1), 161-166
[8] Rui, L., Xie, M., Hu, B., Zhou, L., Yin, D., Zeng, X. (2017). A comparative study on chitosan/gelatin composite films with conjugated or incorporated gallic acid. Carbohyd Polym., 173, 473-481.
[9] Arora, A., Padua, G. (2009). Review: Nanocomposites in Food Packaging. Journal of Food Science, 75(1), 43 [8] Azeredo, H. 2009. Nanocomposites for food packaging applications. Food Res. Int., 42(9), 1240-1253
[10] Azeredo, H. (2009). Nanocomposites for food packaging applications. Food Res. Int., 42(9), 1240-1253.
[11] Kam, W. Y. J., Mirhosseini, H., Abas, F., Hussain, N., Hedayatnia, S., Chong, H. L. F. (2018). Antioxidant activity enhancement of biodegradable film as active packaging utilizing crude extract from durian leaf waste. Food Control., 90, 66-72
[12]ASTM. (2002). Standard Test Method for Tensile Properties of Thin Plastic Sheeting. Annual book of ASTM. Philadelphia, PA: American Society for Testing and Material., D 882-02.
[13]ASTM. (2010).Standard Test Methods for Water Vapor Transmission of Materials. Annual book of ASTM.  Philadelphia, PA: American Society for Testing and Materia., Designation: E96/E96. 730-739
[14] Lavorgna, M., Piscitelli, F., Mangiacapra, P., and Buonocore, G. G. (2010). Study of the combined effect of both clay and glycerol plasticizer on the properties of chitosan films. Carbohyd Polym., 82(2), 291-298.
[15] Hanani, Z. N., Roos, Y. H., Kerry, J. P. (2014). Use and application of gelatin as potential biodegradable packaging materials for food products. Int. J. Biol. Macromo., 71, 94-102.
[16]da Trindade Alfaro, A., Fonseca, G. G., Prentice-Hernández, C. (2013). Enhancement of functional properties of wami tilapia (Oreochromis urolepis hornorum)  skin  gelatin  at  different  pH  values.   Food
Bioprocess Tech., 6(8), 2118-2127.
[17] Srinivasa, P. C., Ramesh, M. N., Tharanathan, R. N. (2007). Effect of plasticizers and fatty acids on mechanical and permeability characteristics of chitosan films. Food Hydrocoll., 21(7), 1113-1122.
[18] Cussler, E., Hughes, S., Ward III, W., Aris, R. (1988). Barrier membranes. J. Membrane Sci., 38(2), 161-174.
[19] Ferreira, A. R., Torres, C. A., Freitas, F., Sevrin, C., Grandfils, C., Reis, M. A., Coelhoso, I. M. (2016). Development and characterization of bilayer films of FucoPol and chitosan. Carbohyd polym., 147, 8-15.
[20]Kerch, G., Zicans, J., Meri, R. M.. (2010). The effect of chitosan oligosaccharides on bread staling. J. Cereal Sci., 52(3), 491-495.
[21] Silva-Castro, I., Martín-Ramos, P., Matei, P. M., Fernandes-Correa, M., Hernández-Navarro, S., Martín-Gill, J. (2017). Eco‐Friendly Nanocomposites of Chitosan with Natural Extracts, Antimicrobial Agents, and Nanometals. Handbook of Composites from Renewable Materials, Nanocomposites: Advanced Applications., 8, 35.
[22] Qiao, C., Ma, X., Zhang, J., Yao, J. (2017). Molecular interactions in gelatin/chitosan composite films. Food Chem., 235, 45-50.
[23] Mohammadi, R., Mohammadifar, M. A., Rouhi, M., Kariminejad, M., Mortazavian, A. M., Sadeghi, E., Hasanvand, S. (2018). Physico-mechanical and structural properties of eggshell membrane gelatin-chitosan blend edible films. Int. J. Biol. Macromo., 107, 406-412.
[24] Gómez-Guillén, M. C., Giménez, B., López-Caballero, M. A., Montero, M. P.( 2011). Functional and bioactive properties of collagen and gelatin from alternative sources: A review. Food Hydrocoll., 25(8), 1813-1827.
[25] Atef, M., Rezaei, M., Behrooz, R., (2015), Characterization of physical, mechanical, and antibacterial properties of agar-cellulose bionanocomposite films incorporated with savory essential oil, Food Hydrocoll., 45, 150-157.
[26] Garcı́a, M. A., Pinotti, A., Martino, M. N. Zaritzky, N. E., (2004), Characterization of composite hydrocolloid films, Carbohyd Polym., 56(3), 339-345.
[27]Muyonga, J., Cole, C. G., Duodu, K. (2004). Fourier transform infrared(FTIR)spectroscopic study of acid soluble collagen and gelatin from skins and bones of young and adult Nile perch (Lates niloticus). Food Chem., 86, 325–332.
[28]Pereda, M., Ponce, A. G., Marcovich, N. E., Ruseckaite, R. A., Martucci, J. F. (2011). Chitosan–gelatin composites and bi-layer films with potential antimicrobial activity. Food Hydrocoll., 25, 1372–1381.
Innovative Food Technologies
Volume 6, Issue 1
November 2018
Pages 149-159

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History

  • Receive Date: 13 May 2018
  • Revise Date: 30 June 2018
  • Accept Date: 14 July 2018

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