Comparison of Antioxidant effect of different extracts from Melissa officinalis leaves with immersion and microwave-assisted extractions and its oxidative stability on soybean oil

Document Type : Research Article

Authors

1 Department of Food and Science Technology, Azadshahr Branch, Islamic Azad University, Azadshahr, Iran

2 Department of Chemistry, Azadshahr Branch, Islamic Azad University, Azadshahr, Iran

Abstract

The aim of this study was extraction of phenolic compounds from Melissa officinalis leaves by immersion and microwave-assisted extraction methods and different solvents (water, methanol 80% and ethanol 50%). Total phenolic content of the extracts determined according to the Folin-Ciocalteu method, and the antioxidant activities of them investigated by DPPH radical scavenging, ferric reduction activity potential (FRAP) and total antioxidant capacity assays. In both methods, ethanolic and water extracts had the maximum and minimum of phenolic extraction efficiency, respectively. Results showed increasing in time of microwave exposure, the extraction rate enhanced, but by immersion method, extraction of phenolic compounds until 18 hours increased then decreased. Comparison between two techniques revealed that microwave-assisted extraction technique had higher extraction efficiency than immersion technique. Antioxidant activity of extracts by two mentioned techniques declared that the least EC50 in DPPH radical scavenging activity, reducing power assay, and total antioxidant capacity belonged to ethanolic extract of microwave-assisted method. Finally, microwave-assisted ethanolic extract with best efficiency was added to soybean oil (250, 500 and 1000 ppm) without any antioxidant and compared by adding BHT antioxidant (100 and 200 ppm). The result of Peroxide value and thiobarbituric acid results showed that oxidation inhibited during storage furthermore 1000 ppm of extract was the most effective to inhibit oxidation so could be successfully replaced by BHT.
 

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[1] Yanishlieva-Maslarova, N.V. (2001). Inhibiting oxidation, in: Pokorny, J., Yanishlieva, N., Gorden, M. (Eds.), Antioxidants in Food.Woodhead Publishing Ltd. ,Cambridge, UK, pp 22-70.
[2] Martinez-Tome, M., Jimenez, A., Ruggieri, S., Frega, N., Strabbioli, R., Murcia, M. (2001). Antioxidant properties of mediterranean spices compared with common food additives. J. Food Protec., 64, 1412-1419.
[3] Kahl, R., Kappus, H. (1993). Toxicity of synthetic antioxidants BHT and BHA in comparison with natura antioxidants vitamin E. Z. Lebensm. Unters. Forsch., 196, 329- 338.
[4] Namiki, M. (1990). Antioxidants, antimutagens in food. J. Crit. Rev. Food Sci. Nutr., 29(4), 273-300.
[5] Kulisic, T., Radonic, A., Katalinic, V. (2004). Use of different methods for testing antioxidative of oregano essential oil. J. Food Chem., 85, 633-640.
[6] Koch-Heitzmann, I., Schultze, W. (1984). Melissa officinalis L. an old medicinal plant with new therapeutic action. Dtsc Apothek Ztg., 124, 2137-2145.
[7] Lamaison, J.L., Petitjean-Freytet, C., Duband, F., Carnat, A.P. (1991). Rosemarinic acid content and antioxidant activity of French lamiaceae. Fitoterapia, 62, 166-171.
[8] Patora, J., Klimek, B. (2002). Flavonoids from lemon balm (Melissa officinalis L., Lamiaceae). Acta Pol Pharm., 59(2), 139-143.
[9] De Carvalho, N.C., Correa-Angeloni, M.J., Leffa, D.D., Moreira, J., Nicolau, V., De Aguiar Amaral, P., et al. (2011). Evaluation of the genotoxic and antigenotoxic potential of Melissa officinalis in mice. Genet Mol. Biol., 34(2), 290-297.
 [10] Ibarra, A., Feuillere, N., Roller, M., Lesburgere, E., Beracochea, D. (2010). Effects of chronic administration of Melissa officinalis L. extract on anxiety-like reactivity and on circadian and exploratory activities in mice. Phytomedicine, 17(6), 397-403.
 [11] Buta, N., Popa, N., Roman, L., Bordea, G., Bordea, A., Bordea, N. (2013). The antioxidant effect of Melissa officinalis extract regarding the sunflower oil used in food thermal aplications. J. Agroal. Proc. Technol., 19(2), 276-279.
 [12] Herodez, S.S., Hadolin, M., Skerget, M., Knez, Z. (2003). Solvent extraction study of antioxidants from Balm (Melissa officinalis L.) leaves. Food Chem., 80, 275-282.
[13] Miron, T. L., Herrero, M., Ibáñez, E. (2013). Enrichment of antioxidant compounds from Lemon Balm (Melissa officinalis) by pressurized liquid extraction and enzyme- assisted extraction. J. Chromatogr. A., 1288, 1-9.
[14] Ince, A.E., Sahin, S., Sumnu, S.G. (2013). Extraction of phenolic compounds from melissa using microwave and ultrasound. Turk. J. Agric. For., 37, 69-75.
[15] Rafiee, Z., Jafari, S., Alami, M., Khomeiri, M. (2012). Antioxidant effect of microwave-assisted extracts of olive leaves on sunflower oil. J. Agr. Sci. Tech., 14, 1497-509.
[16] Slinkard, K., Singleton, V.L. (1977). Total phenol analysis; automation and comparison with manual methods. Am. J. Enol. Viticul., 28, 49-55.
 [17] Shimada, K., Fujikawa, K., Yahara, K., Nakamura, T. (1992). Antioxidative properties of xanthin on autoxidation of soybean oil in cyclodextrin emulsion. Agr. Food Chem., 40, 945-948.
 [18] Yildirim, A., Mavi, A., Kara, A.A. (2001). Determination of antioxidant and antimicrobial activities of Rumex crispus L. extracts. Agr. Food Chem., 49, 4083-4089.
[19] Prieto, P., Pineda, M., Aguilar, M. (1999). Spectrophotometric quantitation of antioxidant promising extraction tool for medicinal plant research. J. Food Chem., 92, 144-151.
[20] AOAC. (1990). Official Methods of Analysis. Association of Official Analytical Chemists, 15th ed. Washington, DC., USA.
[21] Pinelo, M., Del Fabbro, P., Marzocco, L., Nunez, M.J., Vicoli, M.C. (2005). Optimization of continuous phenol extraction from Vitis vinifera byproducts. Food Chem., 92, 109-117.
[22] Zhang, Y., Yang, L., Zu, Y., Chen, X., Wang, F., Liu, F. (2010). Oxidative stability of sunflower oil supplemented with carnosic acid compared with synthetic antioxidants during accelerated storage. J. Food Chem., 118(3), 656- 662.
[23] Hemwimon, S., Pavasant, P., Shotipruk, A. (2007). Microwave-assisted extraction of antioxidative anthraquinones from roots of Morinda citrifolia. Sep. Purif. Technol, 54, 44–50.
[24] Pan, X., Niu, G., Liu, H. (2003). Microwave-assisted extraction of tea polyphenols and tea caffeine from green tea leaves. Chem. Eng. Process., 42(2), 129-133.
[25] Barreira, J.C.M., Ferreira, I.C.F.R., Oliveira, M.B.P.P., Pereira, J.A. (2008). Antioxidant activities of the extracts from chestnut flower, leaf, skins and fruit. Food Chem., 107(3), 1106-1113.
[26] Pereira, R.P., Fachinetto, R., De Souza Prestes, A., Puntel, R.L., Da Silva, G.N.S., Heinzmann, B.M. (2009). Antioxidant effects of different extracts from Melissa officinalis, Matricaria recutita and Cymbopogon citratus. Neurochem. Res., 34(5), 973-83.
[27] Pereira, J.A., Oliveira, I., Sousa, A., Valento, P., Andrade, P.B., Ferreira, I.C.F.R., Ferreres, F., Bento, A., Seabra, R., Estevinho, L. (2007). Walnut (Juglans regia L.) leaves: Phenolic compounds, antibacterial activity and antioxidant potential of different cultivars. Food Chem. Toxicol., 45(11), 2287- 2295.
 [28] Olivera, I., Sousa, A., Valentao, P., Andrade, P., Ferreira, F., Bento, A., Seabra, R., Estevinho, L., Pereira, J.A. (2007). Hazel (Corylus avellana) leaves as source of antimicrobial and antioxidant compounds. Food Chem., 105, 1018-1025.
[29] Arabshahi-Delouee, S., Urooj, A. (2007). Antioxidant properties of various solvent extracts of mulberry (Morus indica L.) leaves. Food Chem., 102(4), 1233-1240.
 [30] Chun, S.S., Vattem, D.A., Lin, Y.T., Shetty, K. (2005). Phenolic antioxidants from clonal oregano (Origanum vulgare) with antimicrobial activity against Helicobacter pylori. Process Biochem., 40, 809-816.
[31] Contini, M., Baccelloni, S., Massantini, R., Anelli, G. (2008). Extraction of natural antioxidants from hazelnut (Corylus avellana L.) shell and skin wastes by long maceration at room temperature. Food Chem., 110, 659-669.
[32] Farag, R.S., Mahmoud, E.A., Basuny, A.M. (2007). Use crude olive leaf juice as a natural antioxidant for the stability of sunflower oil during heating. Int. J. Food Sci. Tech., 42, 107-115.
[33] Mohamadi, S., Kiarostami, K., Nazem Bokaii, Z. (2014). The Study of antioxidant property of metanolic extracts of Melissa officinalis L. and Salvia officinalis L. on stability of soybean oil. J. Agroal. Proc. Technol., 20(4), 293-297.