Investigation of antimicrobial, antioxidant and physicochemical properties of active film based on whey protein containing pomegranate and red grape anthocyanins and zinc oxide nanoparticles

Document Type : Research Article


1 Urmia University

2 Department of food science and hygiene, Faculty of Para-veterinary, Ilam University


Introductio: Active anti-microbial packages, metal nanocomposites are a new generation of packages with nano structure, which are produced by direct combination of metal nanoparticles with base resin. This research was conducted with the aim of investigating the physicochemical, antioxidant and antimicrobial properties of whey protein concentrate smart film containing pomegranate and red grape anthocyanins and zinc oxide nanoparticles. Materials and methods: permeability to water vapor, antioxidant properties, antimicrobial properties by disk diffusion method and differential scanning calorimeter test were performed on the prepared films. Agar diffusion method was used to determine the antimicrobial effects of the film. Results and Discussion: By increasing the percentage of zinc oxide nanoparticles and anthocyanin in the film, the percentage of solids in the film increased. The antioxidant activity of active films increased significantly with the increase of anthocyanin content of the films. The highest antioxidant activity with a significant difference (p<0.05) was attributed to the film sample with 2.6 cc extract. According to the data obtained from the DSC test, with the addition of anthocyanins, the temperature of 290 and the glass transition have changed to some extent, and on the other hand, it can be concluded that the addition of nanoparticles to the film can reduce the glass transition temperature. Conclusion: In general, this study showed that anthocyanins and zinc oxide nanoparticles have the potential to be used to prepare films based on bioactive whey concentrate with improved physicochemical properties and biological properties such as antioxidant properties when used in appropriate concentrations.

Graphical Abstract

Investigation of antimicrobial, antioxidant and physicochemical properties of active film based on whey protein containing pomegranate and red grape anthocyanins and zinc oxide nanoparticles


  • The antioxidant activity of the active films increased significantly with increasing anthocyanin content of the films.
  • According to the data obtained from the DSC test, with the addition of anthocyanins, the glass transition temperature have decreased somewhat.
  • Production film based on whey concentrate can be used as a coating for dairy products along with natural antioxidants due to its hydrophilic nature.
  • The results showed that the control sample had little resistance against these two pathogenic bacteria, while the films containing anthocyanin and zinc oxide nanoparticles had antimicrobial properties.


Main Subjects

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