Optimization of hemp (Canabis Sativa L.) seed oil extraction process by ohmic process and response surface methodology

Document Type : Research Article


1 sari agricultural sciences and natural resources university

2 Department of food science and technology,sari agricultural sciences and natural resources university


The hemp (Cannabis sativa L.) plant is as a rich resource of the bioactive compounds.Hempseed oil is well known for its good ratio of unsaturated omega-6 to omega-3 fatty acids and its unique nutrient and medicinal properties.Therefore, the aim of the present study was to optimize the extraction parameters of hempseed oil in order to achieve the maximum levels of oil extraction performance and antioxidant activity and the minimumperoxide value and color index. In the present study, the effects of solvent ratio of hexane to isopropanol (0:100, 50:50, and 100:0 v/v%), voltage (40, 60 and 80 v/cm) and time (5, 10 and 15 minutes) were investigated on oil extraction efficiency, total phenol content, DPPH radical scavenging, peroxide value and oil color index and extraction conditions were optimized by using the RSM and Box-Behnken Design (BBD) metod. The optimal conditions for oil extraction were obtained as hexane to isopropanol ratio of approximately 73 to 27 (v / v%), process time of 8.40 min and voltage of 51.61 V/cm. The R2 value of all responses was also above 0.96, which was a confirmation of these models. Ohmic pre-treatmentcan improve hemp seed oil extraction as a simple and fast method.

Graphical Abstract

Optimization of hemp (Canabis Sativa L.) seed oil extraction process by ohmic  process and response surface methodology


  • Oil extraction from hemp seed was evaluated by ohmic pre-treatment.
  • Response surface methodology was used to optimize regression models to estimate the oil yield, antioxidant activity and oil color.
  • The effect of the solvent ratio of hexane to isopropanol, voltage and extraction time was investigatedon the desired responses.
  • The efficiency, phenol, DPPH inhibition, peroxide, and color of oil were analyzed.
  • The optimal extraction conditions were calculated with a solvent ratio of hexane to isopropanol of approximately 73 to 27 v/v%, time of 8.40 min and voltage of 51.61 v/cm.


Main Subjects

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