Optimization of hemp (Canabis Sativa L.) seed oil extraction process by ohmic process and response surface methodology

Document Type : Research Article

Authors

1 sari agricultural sciences and natural resources university

2 Department of food science and technology,sari agricultural sciences and natural resources university

Abstract

The hemp (Cannabis sativa L.) plant is as a rich resource of the bioactive compounds.Hempseed oil is well known for its good ratio of unsaturated omega-6 to omega-3 fatty acids and its unique nutrient and medicinal properties.Therefore, the aim of the present study was to optimize the extraction parameters of hempseed oil in order to achieve the maximum levels of oil extraction performance and antioxidant activity and the minimumperoxide value and color index. In the present study, the effects of solvent ratio of hexane to isopropanol (0:100, 50:50, and 100:0 v/v%), voltage (40, 60 and 80 v/cm) and time (5, 10 and 15 minutes) were investigated on oil extraction efficiency, total phenol content, DPPH radical scavenging, peroxide value and oil color index and extraction conditions were optimized by using the RSM and Box-Behnken Design (BBD) metod. The optimal conditions for oil extraction were obtained as hexane to isopropanol ratio of approximately 73 to 27 (v / v%), process time of 8.40 min and voltage of 51.61 V/cm. The R2 value of all responses was also above 0.96, which was a confirmation of these models. Ohmic pre-treatmentcan improve hemp seed oil extraction as a simple and fast method.

Graphical Abstract

Optimization of hemp (Canabis Sativa L.) seed oil extraction process by ohmic process and response surface methodology

Highlights

  • Oil extraction from hemp seed was evaluated by ohmic pre-treatment.
  • Response surface methodology was used to optimize regression models to estimate the oil yield, antioxidant activity and oil color.
  • The effect of the solvent ratio of hexane to isopropanol, voltage and extraction time was investigatedon the desired responses.
  • The efficiency, phenol, DPPH inhibition, peroxide, and color of oil were analyzed.
  • The optimal extraction conditions were calculated with a solvent ratio of hexane to isopropanol of approximately 73 to 27 v/v%, time of 8.40 min and voltage of 51.61 v/cm.

Keywords

Main Subjects

References

 
[1] Palade, L. M., Habeanu, M., Marin, D. E., Chedea, V. S., Pistol, G. C., Grosu, I. A., ... & Taranu, I. (2019). Effect of Dietary Hemp Seed on Oxidative Status in Sows during Late Gestation and Lactation and Their Offspring. Anim., 9(4), 194.
[2] Drinić, Z., Vidović, S., Vladić, J., Koren, A., Kiprovski, B., & Sikora, V. (2018). Effect of extraction solvent on total polyphenols content and antioxidant activity of Cannabis sativa L. Lekovite sirovine., (38), 17-21.
[3] Wang, X. S., Tang, C. H., Yang, X. Q., & Gao, W. R. (2008). Characterization, amino acid composition and in vitro digestibility of hemp (Cannabis sativa L.) proteins. Food Chem., 107(1), 11-18.
[4] Oomah, B. D., Busson, M., Godfrey, D. V., & Drover, J. C. (2002). Characteristics of hemp (Cannabis sativa L.) seed oil. Food Chem, 76(1), 33-43.
[5] Yan, X., Tang, J., dos Santos Passos, C., Nurisso, A., Simoes-Pires, C. A., Ji, M., ... & Fan, P. (2015). Characterization of lignanamides from hemp (Cannabis sativa L) seed and their antioxidant and acetylcholinesterase inhibitory activities. J. Agric. Food Chem., 63(49), 10611-10619.
[6] Azadmard-Damirchi, S., Habibi-Nodeh, F., Hesari, J., Nemati, M., & Achachlouei, B. F. (2010). Effect of pretreatment with microwaves on oxidative stability and nutraceuticals content of oil from rapeseed. Food Chem., 121(4), 1211-1215.
[7] Li, H., Pordesimo, L., & Weiss, J. (2004). High intensity ultrasound-assisted extraction of oil from soybeans. Food Res. Int., 37(7), 731-738.
[8] Knirsch, M. C., Dos Santos, C. A., de Oliveira Soares, A. A. M., & Penna, T. C. V. (2010). Ohmic heating–a review. Trends Food Sci Technol., 21(9), 436-441.
[9] Loypimai, P., Moongngarm, A., & Chottanom, P. (2015). Impact of stabilization and extraction methods on chemical quality and bioactive compounds of rice bran oil. EMIR J FOOD AGR., 849-856.
[10] Kumari, K., Mudgal, V. D., Viswasrao, G., & Srivastava, H. (2016). Studies on the effect of ohmic heating on oil recovery and quality of sesame seeds. Int. J. Food Sci., 53(4), 2009-2016.
[11] Hernández-Santos, B., Rodríguez-Miranda, J., Herman-Lara, E., Torruco-Uco, J. G., Carmona-García, R., Juárez-Barrientos, J. M., ... & Martínez-Sánchez, C. E. (2016). Effect of oil extraction assisted by ultrasound on the physicochemical properties and fatty acid profile of pumpkin seed oil (Cucurbita pepo). Ultrason Sonochem., 31, 429-436.
[12] Jalili, F., Jafari, S. M., Emam-Djomeh, Z., Malekjani, N., & Farzaneh, V. (2018). Optimization of ultrasound-assisted extraction of oil from canola seeds with the use of response surface methodology. Food Anal., 11(2), 598-612.
[13] Samaram, S., Mirhosseini, H., Tan, C. P., Ghazali, H. M., Bordbar, S., & Serjouie, A. (2015). Optimisation of ultrasound-assisted extraction of oil from papaya seed by response surface methodology: Oil recovery, radical scavenging antioxidant activity, and oxidation stability. Food Chem., 172, 7-17.
[14] Maghsoudlou, E., Esmaeilzadeh Kenari, R., & Raftani Amiri, Z. (2017). Evaluation of Antioxidant Activity of Fig (Ficus carica) Pulp and Skin Extract and Its Application in Enhancing Oxidative Stability of Canola Oil. J FOOD PROCESS PRES., 41(4), e13077.
[15] Capannesi, C., Palchetti, I., Mascini, M., & Parenti, A. (2000). Electrochemical sensor and biosensor for polyphenols detection in olive oils. Food Chem., 71(4), 553-562.
[16] Li, H. Z., Zhang, Z. J., Xue, J., Cui, L. X., LI, X. J., & CHEN, T. (2016a). Optimization of ultrasound-assisted extraction of phenolic compounds, antioxidants and rosmarinic acid from perilla leaves using response surface methodology. FOOD SCI TECHNOL INT., 36(4), 686-693.
[17] Moghimi, M., Farzaneh, V., & Bakhshabadi, H. (2018). The effect of ultrasound pretreatment on some selected physicochemical properties of black cumin (Nigella Sativa). Nutrire, 43(1), 18.
[18] Li, T., Qu, X. Y., Zhang, Q. A., & Wang, Z. Z. (2012). Ultrasound-assisted extraction and profile characteristics of seed oil from Isatis indigotica Fort. Ind Crops Prod., 35(1), 98-104.
[19] Luque-Garcıa, J. L., & De Castro, M. L. (2004). Ultrasound-assisted soxhlet extraction: an expeditive approach for solid sample treatment: application to the extraction of total fat from oleaginous seeds. J. Chromatogr. A., 1034(1-2), 237-242.
[20] Manach, C., Scalbert, A., Morand, C., Rémésy, C., & Jiménez, L. (2004). Polyphenols: food sources and bioavailability. AM J CLIN NUTR., 79(5), 727-747.
[21] Tabaraki, R., & Nateghi, A. (2011). Optimization of ultrasonic-assisted extraction of natural antioxidants from rice bran using response surface methodology. Ultrason Sonochem., 18(6), 1279-1286.
[22] Moongngarm, A., Loypimai, P., Fitriati, A., & Moontree, T. (2019). Ohmic heating assisted extraction improves the concentrations of phytochemicals in rice bran oil and unsaponifiable matter. Int Food Res J., 26(4).
[23] Horwitz, W., Chichilo, P., & Reynolds, H. (1970). Official methods of analysis of the Association of Official Analytical Chemists. Official Methods of Analysis of The Association of Official Analytical Chemists.
[24] Cissé, M., Sow, A., Poucheret, P., Margout, D., Ayessou, N. C., Faye, P. G., ... & Diop, C. M. G. (2018). Impact of Extraction Method on Physicochemical Characteristics and Antioxidant Potential of Adansonia digitata Oil. FOOD SCI NUTR., 9(08), 937.
[25] Li, T., Xu, J., Wu, H., Wang, G., Dai, S., Fan, J., ... & Xiang, W. (2016b). A saponification method for chlorophyll removal from microalgae biomass as oil feedstock. Mar. Drugs., 14(9), 162.
 
Innovative Food Technologies
Volume 7, Issue 4
August 2020
Pages 581-596

Files

History

  • Receive Date: 27 April 2020
  • Revise Date: 02 July 2020
  • Accept Date: 27 September 2020

Share

How to cite

Statistics