Preparation and characterization of Mentha nanoemulsions using gelatin- polysorbate 20 based multicomponent stabilizer system as food Preservative

Document Type : Research Article

Authors

1 MSC, Department of Chemical Engineering, Tabriz Branch, Islamic Azad University, Tabriz

2 PhD, Assistant professor, Department of chemical engineering,Tabriz Branch, Islamic Azad University,Tabriz

3 PhD, Department of Food Hygiene, Tabriz Branch, Islamic Azad University, Tabriz

Abstract

In this work, the mentha essential oil nanoemulsions were prepared using Tween 20-gelatin multicomponent stabilizing system as food preservative. The response surface methodology (RSM) was applied in order to study the main and interaction effects of the stabilizer components’ proportions, namely, Tween 20 (0-10 gr) and gelatin (0-10 gr) on physicochemical characteristics of obtained nanoemulsions such as average particle size, polydispersity index (PDI), turbidity, conductivity and ztea potential. It was resulted that the response-surface models were significantly (p < 0.05) fitted for studied response variables and could precisely predict all response changes with high coefficients of determination (R2 > 0.90). The overall optimum region with minimum z-average, PDI, turbidity, conductivity and maximum range of zeta-potential was achieved at 8 gr of tween 20 and 2 gr of Gelatin. The optimum mentha essential oil nanoemulsion showed good antibacterial activity against both E. coli and S. areus, alone as well as incorporated in edible coating formulations. The maximum inhibition zone was observed using 1% carboxymethyl, 0.5% glycerol and 5% optimized nanoemulsion.

Graphical Abstract

Preparation and characterization of Mentha nanoemulsions using gelatin- polysorbate 20 based multicomponent stabilizer system as food Preservative

Highlights

  • Nanoemulsion of mentha essential oil with the tween 20 and Gelatin based multicomponent was prepared by solvent replacement method.
  • The optimum nanoemulsions based on RSMwas determined with minimum z-average, PDI, turbidity, conductivity and maximum range of Zeta-potential.
  • The optimum nanoemulsions have antibacterial activity against E. coli and S. areus.
  • The optimum nanoemulsions exhibited the high antioxidant activity.

Keywords

Main Subjects


[1] Panahi, M., Barzegar, H., & Hojjati, M. (2017). Effect of Pistacia atlantica gum oil on antimicrobial and antioxidant properties of edible starch film. Innov. Food Technol., 5, 77-89. [In Persian].
 
[2] Prakash, A., Baskaran, R., Paramasivam, N., & Vadivel, V. (2018). Essential oil based nanoemulsions to improve the microbial quality of minimally processed fruits and vegetables: A review. Food. Res. Int., 111, 509-523.
 
[3] Iqbal, T., Ijaz Hussain, A., Shahzad Chatha, A., Naqvi, A. R., & Bokhari, T. H. (2013). Antioxidant Activity and Volatile and Phenolic Profiles of Essential Oil and Different Extracts of Wild Mint (Mentha longifolia) from the Pakistani Flora, J. Anal. Methods. Chem.,1, 1-6.
 
[4] Beyki, M., & Zhaveh, S. (2014). Encapsulation of Mentha piperita essential oil in chitosan- cinnamon acid nano gel with enhanced antimicrobial activity against Aspergillus flavus, Ind. Crop. Prod., 54, 310-319.
[5] Johnson, M., Wesely, E. G. & Uma, V. (2011). Antibacterial activity of leaves and inter-nodal callus extract of Mentha arvensis, Asian. Pac. J. Trop. Dis., 4, 196-200.
 
[6] Houicher, A., Kuley, E., & Ozogul, F. (2015). Effect of natural extracts mentha spicata L. and Artemisia campestris) on biogenic amine formation of sardine vacuum- packed and refrigerated (Sardina pilchardus) fillets, J. Food Process. Preserv., 39, 2393-2403.
 
[7] Latoch, A., & Dariusz, M. S. (2015). Effect of Mentha piperita on oxidative stability and sensory characteristics of cooked pork sausage, J. Food Process. Preserv., 39, 1566-1573.
[8] Aswathanarayan, J. B., & Vittal, R. R., (2019). Nanoemulsions and Their Potential Applications in Food Industry, Front. Sustain. Food Syst., 3, 1-21.
[9] Zhao, J., Wei, F., Xu, W., & Han, X. (2020). Enhanced antibacterial performance of gelatin/chitosan film containing capsaicin loaded MOFs for food packaging,  Appl. Surf. Sci., 510, 145418.
[10] Schrieber, R., & Careis, H. (2007). Gelatin Handbook-Theory and industrial Practice, WILEY-VCH Verlag GmBH & Co. KGaA.
[11] Tan, C. P., & Nakajima. M. (2005). β-Carotene nanodispersions: preparation, characterization and stability evaluation, Food Chem., 92, 661–671.
 
[12] Anarjan. N., Imededdine, N., & Tan. Ch. P. (2013). Influence of astaxanthin, emulsifier and organic phase concentration on physicochemical properties of astaxanthin nanodispersions, Chem. Cent. J., 7, 1-11.
[13] Alizadeh Asl, S., Mousav, M., & Labbafi, M. (2017). Synthesis and Characterization of Carboxymethyl Cellulose from Sugarcane Bagasse, J. Food Processing Techno., 8, 1-6.
[14] Tian, H., Li, D., Xu, T., Hu, J., Rong, Y., & Zhao, B. (2017). Citral stabilization and characterization of nanoemulsions stabilized by a mixture of gelatin and Tween 20 in an acidic system, J. Sci. Food Agric., 97, 2991-2998.