Effect of temperature on the kinetics of phenolic compounds extraction from ginger (Zingiber officinale) rhizome using ultrasound bath

Document Type : Research Article

Authors

1 Ph.D Student, Dept. of Food science and technology, Sari Agricultural Sciences and Natural Resources University

2 Associate Prof., Dept. of Food science and technology, Sari Agricultural Sciences and Natural Resources University

Abstract

In the present study, the effects of temperatures (40-60 °C) and different times (5-60 min) on polyphenols and flavonoids extraction kinetics using ultrasound bath were investigated, and The IC50 values of DPPH free radical scavenging activity and reducing power of the extracts were calculated. The results showed that the extraction efficiency of phenolic and flavonoid compounds increased with temperature. Also, the highest yield of these compounds were obtained during the first 15 min of extraction, and after that the slope of the extraction decreased. The highest value of phenolic compounds (165.35± 47.69 and 171.13± 36.56 µg GA/g of DW of extract) and flavonoid (9.43± 0.80 and 10.35± 0.93 µg QE/g of DW of extract) were obtained at 15 min, and 50 and 60 ° C, respectively, without significant difference. Therefore, the extraction process can be divided into two stages of rapid extraction and slow extraction. Using the kinetic curves, the maximum efficiency of the antioxidant compounds of ginger rhizome can be found with the least effective time and temperature. In addition, the results showed that ultrasound has a significant effect on the extraction of bioactive compounds from ginger rhizome compared to the traditional method.

Graphical Abstract

Effect of temperature on the kinetics of phenolic compounds extraction from ginger (Zingiber officinale) rhizome using ultrasound bath

Highlights

  • Based on the kinetics graphs, it was determined that the process of ultrasonic extraction can be divided into two phases: rapid extraction and slow extraction.
  • The highest amount of phenolic and flavonoid compounds and the highest antioxidant activity were obtained at the end of the rapid extraction stage and then the results were not significantly different.
  • Ultrasound was more efficient in terms of efficiency and speed of extraction than the traditional method.

Keywords

Main Subjects


 
 [1] Chen, X., Wang, W., Li, S., Xue, J., Fan, L., Sheng, Z. and Chen, Y. (2010). Optimization of ultrasound-assisted extraction of Lingzhi polysaccharides using response surface methodology and its inhibitory effect on cervical cancer cells. Carbohydr. Polym., 80(3), 944-948.
[2] Diem Do, Q., Angkawijaya, A.E., Tran-Nguyen, P. L., Huynh, L. H., Soetaredjo, F. E., Ismadji, S. and Ju, Y.H. (2014). Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophilaaromatica. J FOOD DRUG ANAL., 22, 296- 302.
[3] Chun, S.S., Vattem, D.A., Lin, Y.T. and Shetty, K. (2005). Phenolic antioxidants from clonal oregano (Origanumvulgare) with antimicrobial activity against Helicobacter pylori. Process Biochem., 40(2): 809-816.
[4] Tohma, H., Gülçin, İ., Bursal, E., Gören, A.C., Alwasel, S.H. and Köksal, E. (2017). Antioxidant activity and phenolic compounds of ginger (Zingiber officinale Rosc.) determined by HPLC-MS/MS. J FOOD MEAS CHARACT., 11(2), 556-566.
[5] Huang, W., Xue, A., Niu, H., Jia, Z. and Wang, J. (2009). Optimised ultrasonic-assisted extraction of flavonoids from Folium eucommiae and evaluation of antioxidant activity in multi-test systems in vitro. Food chem., 114(3), 1147-1154.
[6] Hammi, K.M., Jdey, A., Abdelly, C., Majdoub, H. and Ksouri,  R. (2015). Optimization of ultrasound-assisted extraction of antioxidant compounds from Tunisian Zizyphus lotus fruits using response surface methodology. Food chem., 184,80-89.
[7] Hossain, M., Barry-Ryan, C., Martin-Diana, A.B. and Brunton, N. (2011). Optimisation of accelerated solvent extraction of antioxidant compounds from rosemary (Rosmarinus officinalis L.), marjoram (Origanum majorana L.) and oregano (Origanum vulgare L.) using response surface methodology. Food chem., 126,339-346.
[8] Morelli, L.L.L. and Prado, M.A. (2012). Extraction optimization for antioxidant phenolic compounds in red grape jam using ultrasound with a response surface methodology. ULTRASON SONOCHEM., 19, 1144-1149.
[9] Setyaningsih, W., Duros, E., Palma, M. and Barroso, C. (2015). Optimization of the ultrasound-assisted extraction of melatonin from red rice (Oryza sativa) grains through a response surface methodology. Applied Acoustics.
 [10] Ali, A.M.A., El-Nour, M.E.M., and Yagi, S.M. (2018). Total phenolic and flavonoid contents and antioxidant activity of ginger (Zingiber officinale Rosc.) rhizome, callus and callus treated with some elicitors. GENET ENG BIOTECHN N, 16(2), 677-682.
[11] Bharti, I. and Ray, A. (2014). Effect of extraction techniques on total flavonoids, phenolics, and antioxidant activity of different plants extract. Int. j. res. appl., 2(1), 41-48.
[12] Rezaie, M., Farhoosh R., Iranshahi M., Sharif, A. and Golmohamadzadeh, S. (2015). Ultrasonic-assisted extraction of antioxidative compounds from Bene (Pistacia atlantica subsp. mutica) hull using various solvents of different physicochemical properties. Food Chem., 173, 577-583.
[13] Maghsoudlou, E., Esmaeilzadeh Kenari, R., and Raftani Amiri, Z. (2016). Evaluation of Antioxidant Activity of Fig (Ficuscarica) Pulp and Skin Extract and its Application in Enhancing Oxidative Stability of Canola Oil. J FOOD PROCESS PRES., 41(4), 1-11
[14] Sfahlan, A.J., Mahmoodzadeh, A., Hasanzadeh, A., Heidari, R. and Jamei, R. (2009). Antioxidants and antiradicals in almond hull and shell (Amygdalus communis L.) as a function of genotype. Food Chem., 115, 529-533.
[15] Ebrahimzadeh, M.A., Nabavi, S.M., Nabavi, S.F., Bahramian, F. and Bekhradnia, A.R. (2010). Antioxidant and free radical scavenging activity of H. officinalis L. var. angustifolius, V. odorata, B. hyrcana and C. speciosum. PAK J PHARM SCI., 23(1), 29-34.
 [16] Altemimi,A., Choudhary, R., Watson,D.G. and Lightfoot, D.A. (2015). Effects of ultrasonic treatments on the polyphenol and antioxidant content of spinach extracts, ULTRASON SONOCHEM., 24, 247–255.
[17] Silva, E.M., Rogez, H. and Larondelle, Y. (2007). Optimization of extraction of phenolics from Inga edulis leaves using response surface methodology. SEP PURIF TECHNOL., 55(3), 381-387.
 [18] Bey, M.B., Louaileche, H. and Zemouri, S. (2013). Optimization of phenolic compound recovery and antioxidant activity of light and dark dried fig (Ficus carica L.) varieties. Food Sci. Biotechnol., 22(6), 1613-1619.
[19] Liyana-Pathirana, C. and Shahidi, F. (2005). Optimization of extraction of phenolic compounds from wheat using response surface methodology. Food chem., 93(1), 47-56.
[20] Tao, Y., Zhang, Z. and Sun, D.W. (2014). Kinetic modeling of ultrasound-assisted extraction of phenolic compounds from grape marc: influence of acoustic energy density and temperature. ULTRASON SONOCHEM., 21(4), 1461-1469.
[21] Herodez, S.S., Hadolin, M., Skerget, M. and Knez, Z. (2003). Solvent extraction study of antioxidants from Balm (Melissa officinalis L.) leaves, Food Chem., 80, 275–282.
[22] Bidchol, A.M., Wilfred, A., Abhijna, P. and Harish, R. (2011). Free radical scavenging activity of aqueous and ethanolic extract of Brassica oleracea L. var. italica. FOOD BIOPROCESS TECH., 4(7), 1137-1143.
[23] Amarowicz, R., Pegg, R. B., Rahimi-Moghaddam, P., Barl, B. and Weil, J. A. (2004). Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies. Food Chem., 84(4), 551-562.
 [24] Dey, S. and Rathod, V.K. (2013). Ultrasound assisted extraction of β-carotene from Spirulinaplatensis. ULTRASON SONOCHEM., 20(1), 271-276.
[25] Veggi, P.C., Santos, D.T., Fabiano-Tixier, A.S., Le Bourvellec, C., Meireles, M.A.A. and Chemat, F. (2013). Ultrasound-assisted Extraction of Polyphenols from Jatoba (Hymenaea courbaril L. var stilbocarpa) Bark. Food and Public Health, 3(3), 119-129.
[26] Portto, C.D., Porretto, E. and Decorti, D. (2013). Comparison of ultrasound-assisted extrac-tion with conventional extraction methods of oil and polyphenols from grape (Vitis vinifera L.) seeds. ULTRASON SONOCHEM., 20, 1076–1080.