Lipid Extraction from Dunalliela Ultrasonic Waves

Document Type : Research Article


1 Student of master degree, food science and technology, Agricultural institute, Iranian Research Organization for Science and Technology (IROST), Tehran

2 Assistant Professor, Dept. Biosystem engineering, Agricultural research institute, Iranian Research Organization for Science and Technology (IROST), Tehran

3 Associate Professor, Dept. Biosystem engineering, AgriculturalResearchInstitute, Iranian Research Organization for Science and Technology (IROST) , Tehran

4 AgriculturalBiotechnology ResearchInstitute of Iran(ABRII)


In this research Dunalliela sp.M1 isolate was cultivated in open pound bio reactor. This isolate was harvested by electrocoagulation method from culture medium. The harvested biomass was dried and desalinated. Lipid extraction process was carried out by soxhelt method and the effect of extraction time (20- 40min), solvent type (Hexane or mixture of hexane-Isopropanol 3:2) and ultrasonic pre-treatment factors on amount of extracted lipid was evaluated. Test results showed that in soxhelt method the hexane and isopropanol solvents (3:2 ratio),during 40 minutes and by applying of ultrasonic pretreatment had the maximum lipid extraction (16.64%). According to statistics analysis increasing of extraction time had significant effect (p<0.05) on amount of microalgae lipid extraction. But increasing of extraction time with pure hexane, has not any effect on amount of extracted lipid.By applying ultrasonic pre-treatment, extracted oil using hexane and isopropanol (3:2) increased by 4%. Fatty acid profile of extracted oil from this strain of microalgae showed that 59 % was saturated fatty acid which palmitic acid (36.62%) and stearic acid (19.02%) form the main portion of the composition.

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