Evaluation of some structural properties and antioxidant activity of protein-based film from bitter vetch seed and its effect on oxidation of sunflower oil

Document Type : Research Article


1 PhD Student of Food Science and Technology, Department of Food Science, College of Agriculture, Isfahan University of Technology, Isfahan

2 Professors of Food Science and Technology, Department of Food Science, College of Agriculture, Isfahan University of Technology, Isfahan

3 Assisttant Professor of Food Science and Technology, Department of Food Science, College of Agriculture, Isfahan University of Technology, Isfahan


The aim of the present study was to investigate some structural properties and antioxidant activity of a biodegradable film prepared from bitter vetch seed protein and its effect on oxidation of sunflower oil. At first, the film was cast from bitter vetch protein concentrate (BPC) and glycerol. Then structural characteristics of the film were analyzed using fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM) and scanning electron microscopy (SEM). In the next step, the antioxidant activity of the prepared film was measured. The resulting film with specific dimensions was added to sunflower oil samples and its effect on oxidation (peroxide, acidic and thiobarbitoric acid values) of sunflower oil was evaluated in comparison to control sample (without film) in a 45-day storage period at 40 ° C. The infrared spectra showed distinct peaks corresponding to the groups involved in the film structure. AFM and SEM images also revealed the relatively smooth surface (surface roughness of 10.63 nm) and relatively dense and interconnected structure of the films. The antioxidant activity of the prepared film in this study (antioxidant activity 22.56%) was comparable to or even better than other biodegradable films. In the presence of protein film, the oxidation of sunflower oil significantly decreased in comparison with the control (p< 0.001). According to the results, the bitter vetch protein film due to the relatively good antioxidant activity had a positive effect on reducing the oxidation of sunflower oil. Additionally, if some modifications are applied, particularly the water vapor permeability, it will be suitable for use in food packaging.


Main Subjects

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