Comparing the effect of Nepeta glomerulosa essential-oil and its nanoemulsion on quality properties of mayonnaise

Document Type : Research Article


1 Assistant Professor, Department of Food Science and Technology, Sarvestan Branch, Islamic Azad University, Sarvestan, Fars, Iran.

2 M.Sc. graduated, Department of Food Science and Technology, Sarvestan Branch, Islamic Azad University, Sarvestan, Fars, Iran


The current work aims to compare the effect of essential oil (pure and emulsified) Nepeta glomerulosa as natural preservatives for improving the shelf life of high-fat mayonnaise during 6 weeks of storage at 35 ºC. The nanoemulsion of Nepeta glomerulosa essential oil was prepared by the ultrasound cavitation method and physical properties were determined. Soy oil was substituted by 0.5 %w/w of the Nepeta essential oil or its nanoemulsion to compare the effect of natural and chemical (BHT and sodium-benzoate/potassium-sorbate) preservatives on the chemical (pH, acidity, peroxide-value, thiobarbituric acid-reactive-substances), microbial (acid-resistant bacteria, heterofermentative lactic acid bacteria, Escherichia coli, mold and yeast content, and salmonella) and sensory properties of mayonnaise. 1,8-Cineole (26.9%), α-Pinene (7.3%), and Limonene (6.8%) were the main compounds of this essential oil. The mean droplet diameter, PDI and viscosity of Nepeta nanoemulsions were 191.95 nm, 0.255, and 1.03 cP, respectively. The addition of the essential oil (pure or emulsified) significantly improved the oxidative stability of mayonnaise. There was no significant difference between the antioxidant activity of BHT and the essential oil. Over time, a decreasing and increasing trend was observed in pH and acidity, respectively, the slope of these trends was greater in the control sample. Nepeta essential oil and benzoate/sorbate significantly delayed microbial spoilage. The antimicrobial potential of the essential oil and its nanoemulsion were comparable with benzoate/sorbate activity (P > 0.05). At the end of storage, the overall-sensory scores of the sample containing the nanoemulsion were higher than the threshold-limit (>3) while in the 5th week, the control sample was introduced as unacceptable by evaluators. Nepeta nanoemulsion showed good potential to enhance the shelf-life of mayonnaise. Using essential oil in the form of nanoemulsion, not only reduced the consumption of essential oil but the bioactivity of the essential oil was protected for a longer time.

Graphical Abstract

Comparing the effect of Nepeta glomerulosa essential-oil and its nanoemulsion on quality properties of mayonnaise


  • Physical stability of the nanoemulsion of Nepeta glomerulosa essential oil prepared by ultrasound cavitation
  • The suitable antioxidant performance of Nepeta glomerulosa essential oil (pure and emulsified) as compared to chemical preservatives (BHT)
  • Comparable antimicrobial activity of Nepeta glomerulosa essential oil (pure and emulsified) with sodium benzoate-potassium sorbate
  • The positive effect of essential oil and nanoemulsion of massive poinsettia on the sensory characteristics and shelf life of mayonnaise.
  • suitable protective effect of nanoemulsion of Nepeta glomerulosaat a lower concentration of essential oil on shelf-life of mayonnaise


Main Subjects

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Volume 10, Issue 3
May 2023
Pages 229-248
  • Receive Date: 26 September 2022
  • Revise Date: 20 November 2022
  • Accept Date: 30 November 2022
  • First Publish Date: 30 November 2022