Fabrication and implementation of a biosensor for health evaluation in raw milk

Document Type : Research Article

Authors

1 Ph.D. student, Department of Mechanical Engineering Biosystems, Faculty of Agriculture, Shahrekord University, Shahrekord, Iran

2 shahrekord university

3 Shahrekord University.

4 Associate professor, Shahrekord University

5 Professor, Department of Chemistry, Isfahan University of Technology.

6 Associate Professor, Department of Health and Food Quality Control, Faculty of Veterinary Medicine, Shahrekord University.

Abstract

The use of veterinary drugs, including antibiotics, leads to their aggregation in livestock products such as meat, milk, and eggs. Therefore, rapid, accurate and economy detection of antibiotics for monitoring of food security is necessary. In the current research, a biosensor based on pencil graphite electrode modified with grapheme and gold nanoparticles for detection of β-lactam antibiotic in milk samples was fabricated. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques for evaluation of surface electrode changes were applied. Under optimal conditions, using the EIS method specified that in the ranges of 1×10-15 to 1×10-5 M, The relationship between charge transfer resistance and the logarithm of antibiotic concentration was linear and the regression equation was y=119x+2600 (R²=0.977). The current peaks in three measures were achieved as 18, 19.5 and 19 µA. calculation of relative standard deviation (RSD=3.31%) that indicated an acceptable repeatability for biosensor. For investigating of reproducibility, RDS for current peaks was 8.94% that showed a good reproducibility for biosensor. The selectivity of a biosensor in the presence of three antibiotics including streptomycin, tetracycline, and sulfadiazine was checked. Data of charge transfer resistance was indicated that biosensor has non-significant the response towards the other antibiotics. The results showed that biosensor response decreased by 6% after 18 days compared with the first day, which indicates good stability for biosensor. The percentage recovery range for milk samples at concentrations of 1 × 10-9 and 1× 10-11 M for two samples of cow and sheep milk was obtained as 92.3 to 104% in three replications, which indicates an acceptable recovery range for proposed biosensor.

Graphical Abstract

Fabrication and implementation of a biosensor for health evaluation in raw milk

Highlights

  • Nanomaterials based on reduced graphene oxide and gold nanoparticles were used for beta-lactame (BL) detection.
  • The proposed biosensor had high sensitive (LOD: 0.8 fM). 
  • The biosensor indicates a good efficiency for determination of BL in cow and sheep milk samples.

Keywords

Main Subjects

References

[1]     Akbari Kishi S. Asmar M.  and Mirpur M.S. (2017).The Study of Antibiotic Residues in Raw and Pasteurized Milk in Gilan Province . Iran J Med Microbiol. 11 (3) :71-77 (In Persian).
[2]     Wang X. Dong S. Gai P. Duan R., and Li F. (2016). Highly sensitive homogeneous electrochemical aptasensor for antibiotic residues detection based on dual recycling amplification strategy. Biosensors and Bioelectronics, 82, pp 49-54.
[3]     Chen, M. Gan N. Zhou Y. Li T. Xu Q. Cao Y. and Chen Y. (2016). An electrochemical aptasensor for multiplex antibiotics detection based on metal ions doped nanoscale MOFs as signal tracers and RecJf exonuclease-assisted targets recycling amplification. Talanta, 161, pp 867-874.
[4]     Cacciatore G. Petz M. Rachid S. Hakenbeck R. and Bergwerff A. A. (2004). Development of an optical biosensor assay for detection of β-lactam antibiotics in milk using the penicillin-binding protein 2x. Analytica chimica acta, 520(1-2), pp 105-115.
[5]     Moudgil P. Bedi J. S. Aulakh R. S. Gill J. P. S. and Kumar A. (2019). Validation of HPLC Multi-residue Method for Determination of Fluoroquinolones, Tetracycline, Sulphonamides and Chloramphenicol Residues in Bovine Milk. Food Analytical Methods, 12(2), pp 338-346.
[6]     Xu M. Wang R. and Li Y. (2017). Electrochemical biosensors for rapid detection of Escherichia coli O157: H7. Talanta, 162, pp 511-522.
[7]     Jiang, D. Ge P. Wang L. Jiang H. Yang M. Yuan L. and Ju X. (2019). A novel electrochemical mast cell-based paper biosensor for the rapid detection of milk allergen casein. Biosensors and Bioelectronics, 130, pp 299-306.
[8]     SongY. Luo Y. Zhu C. Li H. Du D. and Lin Y. (2016). Recent advances in electrochemical biosensors based on graphene two-dimensional nanomaterials. Biosensors and Bioelectronics, 76, pp 195-212.
[9]     Chen X. Liu Y. Fang X. Li Z. Pu H. Chang J. and Mao S. (2019). Ultratrace antibiotic sensing using aptamer/graphene-based field-effect transistors. Biosensors and Bioelectronics, 126, pp 664-671.
[10] Gupta P. Bharti A. Kaur N. Singh S. and Prabhakar N. (2018). An electrochemical aptasensor based on gold nanoparticles and graphene oxide doped poly (3, 4-ethylenedioxythiophene) nanocomposite for detection of MUC1. Journal of Electroanalytical Chemistry, 813, pp 102-108.
[11] Wu B. Y. Hou S. H. Yin F. Li J. Zhao Z. X. Huang J. D. and Chen Q. (2007). Amperometric glucose biosensor based on layer-by-layer assembly of multilayer films composed of chitosan, gold nanoparticles and glucose oxidase modified Pt electrode. Biosensors and Bioelectronics, 22(6), pp 838-844.
[12] Jin H. Zhao C. Gui R. Gao X. and Wang Z. (2018). Reduced graphene oxide/nile blue/gold nanoparticles complex-modified glassy carbon electrode used as a sensitive and label-free aptasensor for ratiometric electrochemical sensing of dopamine. Analytica chimica acta, 1025, pp 154-162.
[13] Okoth O. K. Yan K. Feng J. and Zhang J. (2018). Label-free photoelectrochemical aptasensing of diclofenac based on gold nanoparticles and graphene-doped CdS. Sensors and Actuators B: Chemical, 256, 334-341.
[14] Li G. Li S. Wang Z. Xue Y. Dong C. Zeng J. and Zhou Z. (2018). Label-free electrochemical aptasensor for detection of alpha-fetoprotein based on AFP-aptamer and thionin/reduced graphene oxide/gold nanoparticles. Analytical biochemistry, 547, pp 37-44.
[15] Rezaei B. Jamei H. R., and Ensafi A. A. (2018). An ultrasensitive and selective electrochemical aptasensor based on RGO-MWCNTs/Chitosan/carbon quantum dot for the detection of lysozyme. Biosensors and Bioelectronics, 115, pp 37-44.
[16] Ciucci F. (2018). Modeling Electrochemical Impedance Spectroscopy. Current Opinion in Electrochemistry, 13, pp 132-139.
[17] Izadi Z. Sheikh-Zeinoddin M. Ensafi A. A. and Soleimanian-Zad S. (2016). Fabrication of an electrochemical DNA-based biosensor for Bacillus cereus detection in milk and infant formula. Biosensors and Bioelectronics, 80, pp 582-589.
[18] Wang N. Lin M. Dai H and Ma H. (2016). Functionalized gold nanoparticles/reduced graphene oxide nanocomposites for ultrasensitive electrochemical sensing of mercury ions based on thymine–mercury–thymine structure. Biosensors and Bioelectronics, 79, pp 320-326.
[19] Ugar M. Tufan A. N. Altun M. Guclu K. and Ozyurek M. (2018). Glutathione Peroxidase Activity of Biological Samples Using a Novel Microplate-Based Method. Current Analytical Chemistry, 14(5), pp 512-518.
[20] Zhao, J., Guo, W., Pei, M., & Ding, F. (2016). GR–Fe3O4 NPs and PEDOT–AuNPs composite based electrochemical aptasensor for the sensitive detection of penicillin. Analytical Methods, 8(22), 4391-4397.
[21] Song, K. M., Jeong, E., Jeon, W., Cho, M., & Ban, C. (2012). Aptasensor for ampicillin using gold nanoparticle based dual fluorescence–colorimetric methods. Analytical and bioanalytical chemistry, 402(6), 2153-2161.
Innovative Food Technologies
Volume 8, Issue 4
August 2021
Pages 457-468

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History

  • Receive Date: 29 April 2019
  • Revise Date: 08 June 2019
  • Accept Date: 20 July 2019

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