Effects of Gamma Irradiation on Microbial and Sensory Properties of Fresh Dill and Savory

Document Type : Research Article

Authors

1 Professor Assistant, Radiation Processing and Dosimetry Research Group, Research School of Radiation Applications, Nuclear Science and Technology Research Institute

2 Research Staff, Radiation Processing and Dosimetry Research Group, Research School of Radiation Applications, Nuclear Science and Technology Research Institute

Abstract

< p >Vegetables play an important role in promoving health and preventing from diseases and they are good carriers to transmit bacteria, parasites, viruses and pathogens to humans. The use of vegetables disinfectants such as chlorine, with their own special risks, is a ways of preventing diseases. Shelf life of these products is limited, after three days at refrigerator, they will lose their consumption and will change to bulk of waste. Irradiation can be a practical treatment to increase safety and shelf-life and exporting of fresh vegetables. In this study, the effects of gamma irradiation doses of 0, 0.25, 0.5, 0.75 and 1 kGy on microbial and sensory qualities of fresh dill and savory at ambient temperature and 4 °C were investigated. According to the statistical results (p < 0.05), there was a significant difference the mean of two treatments of main effects of dose, temperature and time, and interaction of dose*time and time*temperature. The interaction of dose*temperature on mold and yeast count was not significant. The effect of time*temperature*dose was not significant on microbial tests. In sensory-taste tests, there was a statistically significant difference between the mean of two main effects of storage time on the parameters of softness, shape, taste, aroma and color. There was no statistically significant difference between other treatments for measured parameters. According to results samples which were stored at ambient temperature due to high microbial load in first two days were removed of study. In irradiated samples, until the eighth day, a dose-dependent reduction of microbial load was observed. Foodborne pathogenic bacteria including E.coli and Staphylococcus aureus were present in control and 0.25 kGy irradiated samples. Considering acceptable results of microbial and sensory assays, gamma irradiation at dose of 0.5 kGy at 4 °C, can enhance shelf life to 8 days and decrease fresh dill and savory wastes.

Graphical Abstract

Effects of Gamma Irradiation on Microbial and Sensory Properties of Fresh Dill and Savory

Highlights

  • Irradiation is a practical treatment for safety and increasing the shelf life of fresh vegetables and the possibility of exporting to international markets.
  • Samples stored at room temperature were removed from the study cycle in the first two days due to high microbial load.
  • In irradiated samples up to the eighth day of storage, a trend of dose-dependent microbial load was observed.
  • Dietary pathogenic bacteria Escherichia coli and Staphylococcus aureus were present in control and irradiated at 0.25 kGy samples.
  • 5- In terms of microbial and sensory-taste safety, 0.5 kGy irradiation and refrigerated storage increase fresh savory and dill shelf life up to 8 days.

Keywords

Main Subjects


[1] Mahdavianmehr, H., Asnoashari, M., & Sedaghat, N. (2013). New packaging methods of fresh-cut fruits and vegetables. Sci. Quarterly J. Pack. Sci. Tech., 13, 30-43. [In Persian]
[2] Bahraini, M., Habibi Najaf, M.B., M.R. Bassami, Abbaszadegan, M., Bahrami, A.R., & Ejtehadi, H. (2011). Microbial Load Evaluation of Fresh-Cut Vegetables During Processing Steps in A Vegetable Processing Plant Using Minimally Processing Approach. Iran Food Sci. Technol. Res. J., 7(3), 235-242. [In Persian]
[3] Beuchat, L.R. (1996). Pathogenic microorganism associated with fresh product. J. Food Protect., 59, 204-216.
[4] Yousuof, B., Deshi, V., Ozturk, B., & Siddiqui, M.W. (2020). 1 - Fresh-cut fruits and vegetables: Quality issues and safety concerns. Technologies and Mechanisms for Safety Control, 1-15.
[5] Androvic, F. (2012). Gamma Radiation. In: A.K. Kilonzo-Nthenge. Gamma Irradiation for Fresh Produce (1st ed., pp. 251-262). Rijeka: Intech.
[6] UW Food Irradiation Education Group. The Facts About Food Irradiation, 2016. https://uwfood irradiation.engr.wisc.edu/Facts.html.
[7] Almasi, A. (2011). The need to review the hygienic guidelines for disinfecting fruits and vegetables using chlorine. J Kermanshah Univ Med Sci., 3, 231-232. [In Persian]
[8] Deng, L.Zh., Mujumdar, A.S., Pan, Zh., Vidyarthi, S.K., Xu, J., Zielinska, M., & Xiao, H.W. (2020). Emerging chemical and physical disinfection technologies of fruits and vegetables: a comprehensive review. Crit Rev Food Sci Nutr., 60(15), 2481-2508.
[9] Diehl, J.F. (2002). Food irradiation—past, present and future. Radiat. Phys. Chem., 63, (3-6), 211-215.
[10] Catunescu, G.M., Muntean, M., Marian, O., & Paula David, A. (2019). Comparative effect of gamma irradiation, drying and freezing on sensory, and hygienic quality of parsley leaves. LWT - Food Sci. Technol., 115, 108448.
[11] Al-Suhaibani, A.M., Amal N., & Al-Kuraieef, N. (2011). Antioxidant, Microbial and Sensory Evaluation of Fresh Mint Leaves Irradiated with Various Doses of γ-Irradiation. Middle East J. Appl. Sci., 3(4), 122-128.
[12] ISIRI. Microbiology of the food chain —Horizontal method for the enumeration of microorganisms — Part 1: Colony count at 30 °C by the pour plate technique. Document number: 5272-1, 1st.Edition. Institute of Standards and Industrial Research of Iran, Islamic Republic of Iran, 2015. [In Persian]
[13] ISIRI. Microbiology of the food chain —Horizontal method for the enumeration of microorganisms — Part 2: Colony count at 30 °C by the surface plating technique. Document number: 5272-2, 1st.Edition. Institute of Standards and Industrial Research of Iran, Islamic Republic of Iran, 2015. [In Persian]
[14] ISIRI. Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination Part 1: General rules for the preparation of the initial suspension and decimal dilutions. Document number: 8923-1, 1st.Edition. Institute of Standards and Industrial Research of Iran, Islamic Republic of Iran, 2018. [In Persian]
[15] ISIRI. Microbiology of food, animal feed andwater — Preparation, production, storage and performance testing ofculture media. Document number: 8663, 1st.Edition. Institute of Standards and Industrial Research of Iran, Islamic Republic of Iran, 2015. [In Persian]
[16] ISIRI. Microbiology of spices – Specifications. Document number: 3677, first revision. Institute of Standards and Industrial Research of Iran, Islamic Republic of Iran, 2008b. [In Persian]
[17] ISIRI. Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of yeasts and moulds - Part 1: Colony count technique in products with water activity greater than 0.95. Ducument number: 10899-1, first edition. Institute of Standards and Industrial Research of Iran, Islamic Republic of Iran, 2008a. [In Persian]
[18] ISIRI. Microbiology of food and animal feeding stuffs – Horizontal method for the detection and enumeration of coliforms – Most probable number technique. Ducument number: 11166, first edition. Institute of Standards and Industrial Research of Iran, Islamic Republic of Iran, 2008. [In Persian]
[19] ISIRI. Microbiology of food and animal feeding stuffs -Detection and enumeration of presumptive Escherichia coli -Most probable number technique. Document number: 2946, second revision: Institute of Standards and Industrial Research of Iran, Islamic Republic of Iran, 2005. [In Persian]
[20] ISIRI. Microbiology of food and animal feeding stuffs – Enumeration of coagulase – Positive staphylococci (staphylococcus aureus and other species) – Test method Part 1: Technique using baird – parker agar medium Document number: 8606-1, second revision: Institute of Standards and Industrial Research of Iran, Islamic Republic of Iran, 2005. [In Persian]
[21] Methodology of Merck, Germany, 12 (2005).
[22] Serdaroglu, M., & Ozsumer, M. S. (2003). Effects of Soy protein, Why Powder and Wheat Gluten on Quality Characteristics of Cooked Beef Sausages Formulated with 5, 10 and 20% Fat. Electronic J. of Polish Agricultural Universities (EJPAU), Food Sci. Technol., 6(2), 1-9.
[23] Kamat, A., Pingulkar, K., Bhushan, B., Gholap, A., & Thomas, P. (2003). Potential application of low dose gamma irradiation to improve the microbiological safety of fresh coriander leaves. Food Control., 14, 529-537.
[24] Hsu, W.Y., Simonne, A., Jitareerat, P., & Marshal, M.R. (2010). Low-Dose Irradiation Improves Microbial Quality and Shelf Life of Fresh Mint (Mentha piperita L.) without Compromising Visual Quality, J. Food Sci., 75(4), M 222-230.
[25] Al-Suhaibani, A.M., & Al-Kuraieef, A.N. (2016). The effects of gamma irradiation on the microbiological quality, sensory evaluation and antioxidant activity of spinach. Int. J. Chem.Tech. Res., 9(6), 39-47.
[26] Kim, H.J., Feng, H., Toshkov, S.A., & Fan, X. (2005). Effect of Sequential Treatment of Warm Water Dip and Low-dose Gamma Irradiation on the Quality of Fresh-cut Green Onions. J. Food Sci., 70(3), M 179-185.
[27] Matossian, M. (2008). Gamma Irradiation Studies of Spinach Leaves. In: California State Science Fair 2008 Project Summary, Project Number: S1412.
[28] Jadhav, p., Chappalwar, V.M., Bhoyar, A.F., & Chappalwar, A.M. (2013). Study the Shelf Life Extension of Leafy vegetables by Ionizing Radiation. Int. J. Eng. Res. Technol., 2(10), 817-826.
[29] Memon, N., Gat, Y., Arya, Sh., & Waghmare, R. (2020). Combined effect of chemical preservative and different doses of irradiation on green onions to enhance shelf life. J. Saudi Soc. Agri. Sci., 19(3), 207-215.
[30] Segsarnviriya, S. Malakrong, A., & Kongratarpon, T. (2005). The effect of gamma radiation on quality of fresh vegetables. In: International Symposium New Frontier of Irradiated food and Non-Food Products. King Mongkut’s University of Technology Thonburi (KMUTT), Bangkok, Thailand. /http://filing.fda.moph.go.th/library/e-learning/fcdISNF/menu.htmls.