Multi-satge Solvent Extraction of soybean isoflavones

Document Type : Research Article

Authors

1 Associate Professor, Organic Chemistry, Department of Chemical Technologies, Iranian Research Organization for Science and Technology (IROST), Tehran

2 Assistant Professor, Organic Chemistry, Department of Chemical Technologies, Iranian Research Organization for Science and Technology (IROST), Tehran

3 Instructor, Chemistry, Department of Chemical Technologies, Iranian Research Organization for Science and Technology (IROST), Tehran

Abstract

Soya (max Glycine) is one of the enrich source of phytoesterogenes. Isoflavones have a similar structure and function like estrogen in reducing menopausal symptoms and their positive impact have been proved. In the present study extraction of isoflavones from soybeans with ethanol 96% by four different techniques including soxhlet, multi-satge solvent extraction, pre-treated sample by ultrasound and microwave proir to multi-satge solvent extraction carried out and efficient parameters in each methods were optimized. The results showed Soxhlet extraction of 50 g the sample with 150 ml ethanol 96%, during 6 h the maximum extraction yield of phenolics and isoflavanoids were obtained 68.62% and 62.02% respectively. Multi-stage solvent extraction performed by experimental conditions the ratio of solids to solvent extraction 3/10 mg.ml-1, 45C temperature and extraction time 60 min (per extraction), so in this conditions the extraction yield were same as Soxhlet method. In pretreated samples by ultrasound proir to three-stage solvent extraction (for 40 min in cycle 1 and amplitude 60) and microwave-pretreated samples (time leaching of 120 min and radiation time of 5 min), the maximum extraction yield compare to soxhlet method was obtained in solid to solvent ratio of 3.10 mg.ml-1, temperature of 40ºC, extraction time of 15 min. Also Qualitative and quantitative analysis of flavonoids genistein and daidzein in alcoholic dry extract was determined by HPLC.

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References

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Innovative Food Technologies
Volume 4, Issue 4
July 2017
Pages 93-107

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History

  • Receive Date: 01 March 2017
  • Revise Date: 18 July 2017
  • Accept Date: 02 August 2017

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