Iranian Research Organization for Science and Technology (IROST) Innovative Food Technologies 2783-350X 5 3 2018 04 21 Fed-batch Lipid and Carotenoid Production by Rhodotrula glutinis from Pretreated sugarcane bagasse Fed-batch Lipid and Carotenoid Production by Rhodotrula glutinis from Pretreated sugarcane bagasse 397 414 593 10.22104/jift.2017.2421.1563 FA Maryam Banikhaled MSc student, Department of food engineering, Ahwaz Branch, Islamic Azad University, Ahwaz, Khoozestan, Iran Nima Nasirian Assistant professor, Department of Agricultural Mechanization and Biosystem Engineering, Faculty of Agriculture, Shoushtar Branch, Islamic Azad University, Shoushtar Iran Roya Zekavati Assistant professor, Department of Microbiology, faculty of nursing and midwifery, Ahwaz Branch, Islamic Azad University, Ahwaz, Khoozestan, Iran Journal Article 2017 08 20 Lipid and carotenoid synthesis from cheap and abundant lignocellulosic resources has received increased attention in the past decade. This research explores the application of reducing sugar from sugarcane bagasse pretreated with nitric acid for the co-production of lipid and carotenoid by yeast, Rhodotorula glutinis. In this study, the influence of heating time (5-180 min) and nitric acid concentration (0.5-10%) on pretreatment of sugarcane bagasse (lignin reduction) and hydrolysis (fermentable sugars) was examined at 121 °C and the optimized values were determined by response surface methodology (RSM). Subsequently, lipid and carotenoid production by Rhodotrula glutins were scrutinized from the acquired substrate of acid hydrolysis and separate hydrolysis and fermentation (SHF) processes at 25 °C, pH=5.5 during 72 hours post inoculation. Once acid concentration and heating time were 6.67% and 118.75 min, respectively, the optimized value of lignin reduction of 93.4% was obtained. In addition, maximum value of 15.57 g/L of reducing sugar was achieved when the acid concentration and heating time were 0.5% and 180 min, respectively. The lipid content in glucose fed-culture, hydrolysate medium and SHF bioprocess were 27.33±1.06%, 19.52±1.34% and 15.43±0.76% of dry cell weight (dcw), In addition, the carotenoid content of 168±67 µg/g dcw, 99±14 µg/g dcw and 189±13 µg/g dcw, were obtained from corresponding substrates, respectively. The most important fraction of fatty acid methyl esters were palmitic and oleic acid. In addition, from the engineering point of view, the physical properties values of produced lipid from all the substrates, were determined. Lipid and carotenoid synthesis from cheap and abundant lignocellulosic resources has received increased attention in the past decade. This research explores the application of reducing sugar from sugarcane bagasse pretreated with nitric acid for the co-production of lipid and carotenoid by yeast, Rhodotorula glutinis. In this study, the influence of heating time (5-180 min) and nitric acid concentration (0.5-10%) on pretreatment of sugarcane bagasse (lignin reduction) and hydrolysis (fermentable sugars) was examined at 121 °C and the optimized values were determined by response surface methodology (RSM). Subsequently, lipid and carotenoid production by Rhodotrula glutins were scrutinized from the acquired substrate of acid hydrolysis and separate hydrolysis and fermentation (SHF) processes at 25 °C, pH=5.5 during 72 hours post inoculation. Once acid concentration and heating time were 6.67% and 118.75 min, respectively, the optimized value of lignin reduction of 93.4% was obtained. In addition, maximum value of 15.57 g/L of reducing sugar was achieved when the acid concentration and heating time were 0.5% and 180 min, respectively. The lipid content in glucose fed-culture, hydrolysate medium and SHF bioprocess were 27.33±1.06%, 19.52±1.34% and 15.43±0.76% of dry cell weight (dcw), In addition, the carotenoid content of 168±67 µg/g dcw, 99±14 µg/g dcw and 189±13 µg/g dcw, were obtained from corresponding substrates, respectively. The most important fraction of fatty acid methyl esters were palmitic and oleic acid. In addition, from the engineering point of view, the physical properties values of produced lipid from all the substrates, were determined. https://jift.irost.ir/article_593_acc3e0404646c57502b480dc052c4fe1.pdf