Iranian Research Organization for Science and Technology (IROST) Innovative Food Technologies 2783-350X 4 4 2017 06 22 Multi-satge Solvent Extraction of soybean isoflavones Multi-satge Solvent Extraction of soybean isoflavones 93 107 525 10.22104/jift.2017.2047.1462 FA Anvar Shalmashi Associate Professor, Organic Chemistry, Department of Chemical Technologies, Iranian Research Organization for Science and Technology (IROST), Tehran Fereshteh Golmohammad Assistant Professor, Organic Chemistry, Department of Chemical Technologies, Iranian Research Organization for Science and Technology (IROST), Tehran Zeinolabedin Bashiri Sadr Instructor, Chemistry, Department of Chemical Technologies, Iranian Research Organization for Science and Technology (IROST), Tehran Journal Article 2017 03 01 Soya (max Glycine) is one of the enrich source of phytoesterogenes. Isoflavones have a similar structure and function like estrogen in reducing menopausal symptoms and their positive impact have been proved. In the present study extraction of isoflavones from soybeans with ethanol 96% by four different techniques including soxhlet, multi-satge solvent extraction, pre-treated sample by ultrasound and microwave proir to multi-satge solvent extraction carried out and efficient parameters in each methods were optimized. The results showed Soxhlet extraction of 50 g the sample with 150 ml ethanol 96%, during 6 h the maximum extraction yield of phenolics and isoflavanoids were obtained 68.62% and 62.02% respectively. Multi-stage solvent extraction performed by experimental conditions the ratio of solids to solvent extraction 3/10 mg.ml-1, 45C temperature and extraction time 60 min (per extraction), so in this conditions the extraction yield were same as Soxhlet method. In pretreated samples by ultrasound proir to three-stage solvent extraction (for 40 min in cycle 1 and amplitude 60) and microwave-pretreated samples (time leaching of 120 min and radiation time of 5 min), the maximum extraction yield compare to soxhlet method was obtained in solid to solvent ratio of 3.10 mg.ml-1, temperature of 40ºC, extraction time of 15 min. Also Qualitative and quantitative analysis of flavonoids genistein and daidzein in alcoholic dry extract was determined by HPLC. Soya (max Glycine) is one of the enrich source of phytoesterogenes. Isoflavones have a similar structure and function like estrogen in reducing menopausal symptoms and their positive impact have been proved. In the present study extraction of isoflavones from soybeans with ethanol 96% by four different techniques including soxhlet, multi-satge solvent extraction, pre-treated sample by ultrasound and microwave proir to multi-satge solvent extraction carried out and efficient parameters in each methods were optimized. The results showed Soxhlet extraction of 50 g the sample with 150 ml ethanol 96%, during 6 h the maximum extraction yield of phenolics and isoflavanoids were obtained 68.62% and 62.02% respectively. Multi-stage solvent extraction performed by experimental conditions the ratio of solids to solvent extraction 3/10 mg.ml-1, 45C temperature and extraction time 60 min (per extraction), so in this conditions the extraction yield were same as Soxhlet method. In pretreated samples by ultrasound proir to three-stage solvent extraction (for 40 min in cycle 1 and amplitude 60) and microwave-pretreated samples (time leaching of 120 min and radiation time of 5 min), the maximum extraction yield compare to soxhlet method was obtained in solid to solvent ratio of 3.10 mg.ml-1, temperature of 40ºC, extraction time of 15 min. Also Qualitative and quantitative analysis of flavonoids genistein and daidzein in alcoholic dry extract was determined by HPLC. https://jift.irost.ir/article_525_30c2a045cd75671bcb7697e6eec74667.pdf