Iranian Research Organization for Science and Technology (IROST) Innovative Food Technologies 2783-350X 5 1 2017 11 22 Optimization and comparative evaluation of ultrasound-assisted extraction of bioactive phenolic compounds from feijoa (Feijoa sellowiana) leaves Optimization and comparative evaluation of ultrasound-assisted extraction of bioactive phenolic compounds from feijoa (Feijoa sellowiana) leaves 49 64 506 10.22104/jift.2017.506 FA Yasaman Poodi Department of Food Science and Technology, Faculty of Agriculture, University of Zanjan, Zanjan, Iran Mandana Bimakr Assistant Professor, Department of Food Science and Technology, Faculty of Agriculture, University of Zanjan, Zanjan, Iran Ali Ganjloo Department of Food Science and Technology, Faculty of Agriculture, University of Zanjan, Zanjan, Iran Soheila Zarringhalami Department of Food Science and Technology, Faculty of Agriculture, University of Zanjan, Zanjan, Iran Journal Article 2017 05 09 In this study, the effect of ultrasound-assisted extraction (UAE) process independent variables including ultrasonic intensity (714.00, 1425.50 and 2143.00 w cm-2), duty cycle (50, 75 and 100%) and extraction temperature (30, 45 and 60 °C) on feijoa leaves bioactive phenolic compounds was investigated and optimized using response surface methodology based on Box-Behnken design. The total phenolic content of extracts was measured using Folin- Ciocalteu method. Quadratic polynomial model was obtained for total phenolic compounds using multiple linear regression analysis. The optimal conditions were determined as 1749.34 W cm-2 ultrasonic intensity, 86.39% duty cycle and 32.49 °C extraction temperature. The radical scavenging activity of phenolic compounds obtained under optimized UAE conditions was assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2´-Azinobis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) radical scavenging activity methods. Based on results obtained, the total phenolic compound and radical scavenging activity of extract obtained under optimal conditions were 381.20 ± 0.12 mg GAE/g, 81.43 ± 0.10% of DPPH radical inhibition and 86.63 ± 0.26% of ABTS radical inhibition, respectively. The model obtained for prediction of dependent variable has a good closeness with experimental results. The agitated bed extraction technique was investigated to carry out the comparative evaluation. The effect of agitation intensity (50-300 rpm) on total phenolic content of extracts obtained using this technique was studied. Based on results obtained, the highest value of total phenolic content (352.95 ± 0.12 mg GAE/g) could be achieved using 200 rpm agitation intensity. The radical scavenging activity of extract was 73.33 ± 0.16% of DPPH radical inhibition and 82.20 ± 0.12% of ABTS radical inhibition. According to the results obtained it could be stated that ultrasound-assisted extraction is a promising technique to obtain extracts contain more phenolic compounds and higher radical scavenging activity during short extraction time. In this study, the effect of ultrasound-assisted extraction (UAE) process independent variables including ultrasonic intensity (714.00, 1425.50 and 2143.00 w cm-2), duty cycle (50, 75 and 100%) and extraction temperature (30, 45 and 60 °C) on feijoa leaves bioactive phenolic compounds was investigated and optimized using response surface methodology based on Box-Behnken design. The total phenolic content of extracts was measured using Folin- Ciocalteu method. Quadratic polynomial model was obtained for total phenolic compounds using multiple linear regression analysis. The optimal conditions were determined as 1749.34 W cm-2 ultrasonic intensity, 86.39% duty cycle and 32.49 °C extraction temperature. The radical scavenging activity of phenolic compounds obtained under optimized UAE conditions was assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2´-Azinobis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) radical scavenging activity methods. Based on results obtained, the total phenolic compound and radical scavenging activity of extract obtained under optimal conditions were 381.20 ± 0.12 mg GAE/g, 81.43 ± 0.10% of DPPH radical inhibition and 86.63 ± 0.26% of ABTS radical inhibition, respectively. The model obtained for prediction of dependent variable has a good closeness with experimental results. The agitated bed extraction technique was investigated to carry out the comparative evaluation. The effect of agitation intensity (50-300 rpm) on total phenolic content of extracts obtained using this technique was studied. Based on results obtained, the highest value of total phenolic content (352.95 ± 0.12 mg GAE/g) could be achieved using 200 rpm agitation intensity. The radical scavenging activity of extract was 73.33 ± 0.16% of DPPH radical inhibition and 82.20 ± 0.12% of ABTS radical inhibition. According to the results obtained it could be stated that ultrasound-assisted extraction is a promising technique to obtain extracts contain more phenolic compounds and higher radical scavenging activity during short extraction time. https://jift.irost.ir/article_506_42c86460f24e3548b5d7d0e75049a594.pdf